Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Laboratory-acquired infections of Salmonella enterica serotype Typhi in South Africa: Phenotypic and genotypic analysis of isolates
BMC Infectious Diseases, Volume 17, No. 1, Article 656, Year 2017
Notification
URL copied to clipboard!
Description
Background: Workers in clinical microbiology laboratories are exposed to a variety of pathogenic microorganisms. Salmonella species is among the most commonly reported bacterial causes of laboratory-acquired infections. We report on three cases of laboratory-acquired Salmonella enterica serotype Typhi (Salmonella Typhi) infection which occurred over the period 2012 to 2016 in South Africa. Methods: Laboratory investigation included phenotypic and genotypic characterization of isolates. Phenotypic analysis included standard microbiological identification techniques, serotyping and antimicrobial susceptibility testing. Genotypic analysis included the molecular subtyping methodologies of pulsed-field gel electrophoresis analysis, multilocus sequence typing and whole-genome sequencing (WGS); with WGS data analysis including phylogenetic analysis based upon comparison of single nucleotide polymorphism profiles of isolates. Results: All cases of laboratory-acquired infection were most likely the result of lapses in good laboratory practice and laboratory safety. The following critical issues were highlighted. There was misdiagnosis and misreporting of Salmonella Typhi as nontyphoidal Salmonella by a diagnostic laboratory, with associated public health implications. We highlight issues concerning the importance of accurate fluoroquinolone susceptibility testing and interpretation of results according to updated guidelines. We describe potential shortcomings of a single disk susceptibility screening test for fluoroquinolone susceptibility and suggest that confirmatory minimum inhibitory concentration testing should always be performed in cases of invasive Salmonella infections. These antimicrobial susceptibility testing issues resulted in inappropriate ciprofloxacin therapy which may have been responsible for failure in clearance of pathogen from patients. Salmonella Typhi capsular polysaccharide vaccine was not protective in one case, possibly secondarily to a faulty vaccine. Conclusions: Molecular subtyping of isolates proved effective to investigate the genetic relatedness of isolates. Molecular subtyping data interpreted together with epidemiological data allowed us to pinpoint the most likely sources for our cases of laboratory-acquired infection. © 2017 The Author(s).
Authors & Co-Authors
Smith, Anthony Marius
South Africa, Johannesburg
National Health Laboratory Service
South Africa, Johannesburg
University of the Witwatersrand
Smouse, Shannon Lucrecia
South Africa, Johannesburg
National Health Laboratory Service
Tau, Nomsa Pauline
South Africa, Johannesburg
National Health Laboratory Service
South Africa, Johannesburg
University of the Witwatersrand
Bamford, Colleen M.
South Africa, Observatory
Groote Schuur Hospital
South Africa, Cape Town
University of Cape Town
Moodley, Vineshree Mischka
South Africa, Cape Town
Ampath Laboratories
Jacobs, Charlene Ann
South Africa, Cape Town
Communicable Disease Control
McCarthy, Kerrigan Mary
South Africa, Johannesburg
National Health Laboratory Service
Lourens, Adré
South Africa, Tygerberg
Tygerberg Hospital
Keddy, Karen Helena
South Africa, Johannesburg
National Health Laboratory Service
South Africa, Johannesburg
University of the Witwatersrand
South Africa, Sandringham
Nicd
Quan, Vanessa Cecilia
South Africa, Sandringham
Nicd
Nanoo, Ananta
South Africa, Sandringham
Nicd
Gottberg, Anne Von
South Africa, Sandringham
Nicd
Dreyer, Andries William
South Africa, Sandringham
Nicd
Smith, Anthony Marius
South Africa, Sandringham
Nicd
Sooka, Arvinda
South Africa, Sandringham
Nicd
Miller, Cecilia
South Africa, Sandringham
Nicd
Sriruttan, Charlotte
South Africa, Sandringham
Nicd
Cohen, Cheryl D.
South Africa, Sandringham
Nicd
Ihekweazu, Chikwe A.
South Africa, Sandringham
Nicd
von Mollendorf, Claire E.
South Africa, Sandringham
Nicd
Radebe, Frans M.
South Africa, Sandringham
Nicd
Ntshoe, Genevie M.
South Africa, Sandringham
Nicd
Hunt, Gillian M.
South Africa, Sandringham
Nicd
Gouveia, Linda De
South Africa, Sandringham
Nicd
Erasmus, Linda Kathleen
South Africa, Sandringham
Nicd
Smith, Marshagne
South Africa, Sandringham
Nicd
Bodiba, Martha
South Africa, Sandringham
Nicd
Khumalo, Mbhekiseni
South Africa, Sandringham
Nicd
Modise, Motshabi
South Africa, Sandringham
Nicd
Ismail, Nazir Ahmed
South Africa, Sandringham
Nicd
Govender, Nelesh P.
South Africa, Sandringham
Nicd
Page, Nicola A.
South Africa, Sandringham
Nicd
Perovic, Olga
South Africa, Sandringham
Nicd
Murangandi, Oliver
South Africa, Sandringham
Nicd
Crowther-Gibson, Penny
South Africa, Sandringham
Nicd
Mutevedzi, Portia Chipo
South Africa, Sandringham
Nicd
Manesen, Riyadh
South Africa, Sandringham
Nicd
Mpembe, Ruth S.
South Africa, Sandringham
Nicd
Iyaloo, Samantha
South Africa, Sandringham
Nicd
Lengana, Sarona
South Africa, Sandringham
Nicd
Madhi, Shabir A.
South Africa, Sandringham
Nicd
Walaza, Sibongile
South Africa, Sandringham
Nicd
Lindani, Sonwabo
South Africa, Sandringham
Nicd
Meiring, Susan T.
South Africa, Sandringham
Nicd
Motladiile, Thejane
South Africa, Sandringham
Nicd
Chetty, Verushka
South Africa, Sandringham
Nicd
Haumann, Carel
Unknown Affiliation
Hanise, Patricia
Unknown Affiliation
Vasaikar, Sandeep D.
Unknown Affiliation
Black, John M.
Unknown Affiliation
Pearce, Vanessa
Unknown Affiliation
Hoosen, Anwar Ahmed
South Africa
Free State
Kleinhans, Vicky
South Africa
Free State
Karstaedt, Alan S.
Unknown Affiliation
Maluleka, Caroline
Unknown Affiliation
Verwey, Charl
Unknown Affiliation
Feldman, Charles
Unknown Affiliation
Moore, David Paul
Unknown Affiliation
Spencer, David Colin
Unknown Affiliation
Reubenson, Gary
Unknown Affiliation
Swe Swe-Han, Khine Swe Swe
Unknown Affiliation
Wadula, Jeannette
Unknown Affiliation
Nel, Jeremy Stephen
Unknown Affiliation
Lindeque, Kathy
Unknown Affiliation
Nchabeleng, Maphoshane J.
Unknown Affiliation
Plessis, Nicolette Du
Unknown Affiliation
Bosman, Norma
Unknown Affiliation
Kularatne, Ranmini S.
Unknown Affiliation
Lekalakala, R. M.
Unknown Affiliation
Seetharam, Sharona
Unknown Affiliation
Avenant, T.
Unknown Affiliation
Nana, Trusha
Unknown Affiliation
Chibabhai, Vindana
Unknown Affiliation
Maharj, Adhil
Unknown Affiliation
Burra, Asmeeta
Unknown Affiliation
Naby, Fathima
Unknown Affiliation
Dawood, Haiima
Unknown Affiliation
Mlisana, Koleka P.
Unknown Affiliation
Sookan, Lisha
Unknown Affiliation
Ramjathan, Praksha
Unknown Affiliation
Mahabeer, Prasha
Unknown Affiliation
Naidoo, Romola
Unknown Affiliation
Haffejee, Sumayya
Unknown Affiliation
Coovadia, Yacoob Mahomed
Unknown Affiliation
Hamese, Ken
Unknown Affiliation
Sibiya, Ngoaka
Unknown Affiliation
Hoyland, Greta
Unknown Affiliation
Lebudi, Jacob
Unknown Affiliation
Weenink, Eunice
Unknown Affiliation
Abrahams, Riezaah
Unknown Affiliation
Makate, Sindiswa
Unknown Affiliation
Variava, Ebrahim
Unknown Affiliation
Du Plessis, Erna
Unknown Affiliation
Whitelaw, Andrew C.
Unknown Affiliation
Samuel, Catherine Mary
South Africa, Somerset West
Pathcare
Nicol, Mark P.
Unknown Affiliation
Naicker, Preneshni R.
Unknown Affiliation
Abrahams, Shareef
Unknown Affiliation
Brink, Adrian John
South Africa, Durban
Ampath
Prentice, Elizabeth
South Africa, Durban
Ampath
Zietsman, Inge L.
South Africa, Durban
Ampath
Botha, Maria
South Africa, Durban
Ampath
Smith, Peter G.
South Africa, Durban
Ampath
Poswa, Xoliswa
South Africa, Durban
Ampath
Govind, Chetna N.
Unknown Affiliation
Pillay, Keshree
Unknown Affiliation
Budavari, Suzy M.
Unknown Affiliation
Senekal, Marthinus
South Africa, Somerset West
Pathcare
Whitney, Cynthia G.
South Africa, Johannesburg
Cdc
Klugman, K. P.
United States, Atlanta
Emory University
Statistics
Citations: 19
Authors: 110
Affiliations: 13
Identifiers
Doi:
10.1186/s12879-017-2757-2
ISSN:
14712334
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
South Africa