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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Evidence of the role of tick subolesin in gene expression
BMC Genomics, Volume 9, Article 372, Year 2008
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Description
Background: Subolesin is an evolutionary conserved protein that was discovered recently in Ixodes scapularis as a tick protective antigen and has a role in tick blood digestion, reproduction and development. In other organisms, subolesin orthologs may be involved in the control of developmental processes. Because of the profound effect of subolesin knockdown in ticks and other organisms, we hypothesized that subolesin plays a role in gene expression, and therefore affects multiple cellular processes. The objective of this study was to provide evidence for the role of subolesin in gene expression. Results: Two subolesin-interacting proteins were identified and characterized by yeast two-hybrid screen, co-affinity purification and RNA interference (RNAi). The effect of subolesin knockdown on the tick gene expression pattern was characterized by microarray analysis and demonstrated that subolesin RNAi affects the expression of genes involved in multiple cellular pathways. The analysis of subolesin and interacting protein sequences identified regulatory motifs and predicted the presence of conserved protein kinase C (PKC) phosphorylation sites. Conclusion: Collectively, these results provide evidence that subolesinplays a role in gene expression in ticks. © 2008 de la Fuente et al; licensee BioMed Central Ltd.
Authors & Co-Authors
de la Fuente, José Luis
United States, Stillwater
Oklahoma State University
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Maritz-Olivier, Christine
South Africa, Pretoria
University of Pretoria
Naranjo, Victoria
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Ayoubi, Patricia
United States, Stillwater
Oklahoma State University
Nijhof, Ard Menzo
Netherlands, Utrecht
Universiteit Utrecht
Almazán, Consuelo
Mexico, Ciudad Victoria
Universidad Autónoma de Tamaulipas
Canales, Mario
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Pérez de la Lastra, José Manuel
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Galindo, Ruth Cecilia
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Blouin, Edmour F.
United States, Stillwater
Oklahoma State University
Gortázar, Christian
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Jongejan, Frans
Netherlands, Utrecht
Universiteit Utrecht
South Africa, Pretoria
University of Pretoria
Kocan, Katherine M.
United States, Stillwater
Oklahoma State University
Statistics
Citations: 94
Authors: 13
Affiliations: 5
Identifiers
Doi:
10.1186/1471-2164-9-372
e-ISSN:
14712164
Research Areas
Genetics And Genomics