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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
environmental science
Detection and quantification of microcystins from cyanobacteria strains isolated from reservoirs and ponds in Morocco
Environmental Toxicology, Volume 17, No. 1, Year 2002
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Description
In Morocco, the occurrence of toxic cyanobacteria blooms is confirmed in some water bodies used for recreational and/or as drinking water reservoirs. According to WHO recommendations, the establishment of a monitoring program for microcystins is a necessity. This paper presents toxicological studies of 19 toxic cyanobacteria strains of Microcystis, Synechocystis, Pseudanabaena, and Oscillatoria. These strains were isolated from various water bodies including natural lakes, reservoirs, and ponds located in central regions of Morocco. The isolation, culture, and biomass production of these strains was made on Z8 or BG13 media under laboratory controlled conditions. The hepatotoxicity of cyanobacterial lyophilized material was confirmed by mouse bioassays. The amount of microcystins produced by each strain was determined by the enzyme-linked immunosorbent assay (ELISA). The detection and identification of microcystin variants was performed by high performance liquid chromatography (HPLC) with photodiode array detection. Almost all strains showed medium to high toxicity, the estimated LD 50 i.p mice bioassay ranged between 28 to 350 mg/kg body weight. The concentrations of microcystins varied between 2.16 to 944 μg/g and 26.8 to 1884 μg/g dry weight determined by ELISA and HPLC, respectively. The screening of bloom-forming and microcystin producer cyanobacteria strains in these fresh water bodies leads us to propose the need for the establishment of a survey of cyanobacteria and a cyanotoxin-monitoring program. © 2002 by Wiley Periodicals, Inc. Environ Toxicol.
Authors & Co-Authors
Oudra, Brahim
Morocco, Marakech
Faculté Des Sciences Semlalia
Loudiki, Mohammed
Morocco, Marakech
Faculté Des Sciences Semlalia
Vasconcelos, Vítor Manuel Oliveira
Portugal, Porto
Universidade do Porto
Portugal, Matosinhos
Ciimar - Interdisciplinary Centre of Marine and Environmental Research
Sabour, Brahim
Morocco, Marakech
Faculté Des Sciences Semlalia
Sbiyyâa, B.
Morocco, Marakech
Faculté Des Sciences Semlalia
Oufdou, Khalid
Morocco, Marakech
Faculté Des Sciences Semlalia
Mezrioui, Noureddine
Morocco, Marakech
Faculté Des Sciences Semlalia
Statistics
Citations: 111
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1002/tox.10029
ISSN:
15204081
Research Areas
Environmental
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Morocco