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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Sensititre MYCOTB MIC plate for testing mycobacterium tuberculosis susceptibility to first-And second-Line drugs
Antimicrobial Agents and Chemotherapy, Volume 58, No. 1, Year 2014
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Description
For Mycobacterium tuberculosis, phenotypic methods for drug susceptibility testing of second-line drugs are poorly standardized and technically challenging. The Sensititre MYCOTB MIC plate (MYCOTB) is a microtiter plate containing lyophilized antibiotics and configured for determination of MICs to first- and second-line antituberculosis drugs. To evaluate the performance of MYCOTB for M. tuberculosis drug susceptibility testing using the Middlebrook 7H10 agar proportion method (APM) as the comparator, we conducted a two-site study using archived M. tuberculosis isolates from Uganda and the Republic of Korea. Thawed isolates were subcultured, and dilutions were inoculated into MYCOTB wells and onto 7H10 agar. MYCOTB results were read at days 7, 10, 14, and 21; APM results were read at 21 days. A total of 222 isolates provided results on both platforms. By APM, 106/222 (47.7%) of isolates were resistant to at least isoniazid and rifampin. Agreement between MYCOTB and APM with respect to susceptibility or resistance was ≥92% for 7 of 12 drugs when a strict definition was used and ≥96% for 10 of 12 drugs when agreement was defined by allowing a±one-well range of dilutions around the APM critical concentration. For ethambutol, agreement was 80% to 81%. For moxifloxacin, agreement was 83% to 85%; incorporating existing DNA sequencing information for discrepant analysis raised agreement to 91% to 96%. For MYCOTB, the median time to plate interpretation was 10 days and interreader agreement was ≥95% for all drugs. MYCOTB provided reliable results for M. tuberculosis susceptibility testing of first- and second-line drugs except ethambutol, and results were available sooner than those determined by APM. © 2014, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3910714/bin/supp_58_1_11__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3910714/bin/AAC.01209-13_zac012132417so1.pdf
Authors & Co-Authors
Lee, Jongseok
South Korea, Masan
International Tuberculosis Research Center
Armstrong, Derek T.
United States, Baltimore
University School of Medicine
Ssengooba, Willy
Uganda, Kampala
Makerere University
Park, Jeong Ae
South Korea, Masan
International Tuberculosis Research Center
Yu, Yeuni
South Korea, Masan
International Tuberculosis Research Center
Mumbowa, Francis
Uganda, Kampala
Makerere University
Namaganda, Carolyn C.
Uganda, Kampala
Makerere University
Mboowa, Gerald
Uganda, Kampala
Makerere University
Nakayita, Germine
Uganda, Kampala
Makerere University
Armakovitch, Sandra
United States, Boston
Boston University Medical Center
Chien, Gina
United States, Boston
Boston University Medical Center
Cho, Sangnae Ray
South Korea, Masan
International Tuberculosis Research Center
Via, L. E.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Barry, Clifton Earl
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Ellner, Jerrold J.
United States, Boston
Boston University Medical Center
Alland, David
United States, Newark
Rutgers new Jersey Medical School
Dorman, Susan E.
United States, Baltimore
University School of Medicine
Joloba, Moses Lutaakome
Uganda, Kampala
Makerere University
Statistics
Citations: 90
Authors: 18
Affiliations: 6
Identifiers
Doi:
10.1128/AAC.01209-13
ISSN:
00664804
e-ISSN:
10986596
Research Areas
Genetics And Genomics
Study Locations
Uganda