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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Down-regulated claudin-7 immunoexpression in urothelial carcinoma of the urinary bladder
Arab Journal of Urology, Volume 11, No. 2, Year 2013
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Description
Objectives To analyse the gene-expression level of claudin-7 in urothelial carcinoma (UC) of the urinary bladder, and its relationship with clinicopathological variables. Materials and methods This study included 68 specimens of UC of the bladder, comprising 35 with non-muscle-invasive (NMI), stage Ta-T1, and 33 with muscle-invasive (MI) tumours, T2-T4, and 26 of normal urothelium (NU). Total RNA was extracted and 1 μg was reverse transcribed using a cDNA kit. RT-PCR was conducted using SYBR Green I dye to examine the expression levels of the target gene (claudin-7) and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase. Using confocal-laser scanning light microscopy, immunohistochemistry (IHC) was used to validate the RT-PCR data. The correlation between claudin-7 and the clinicopathological variables was assessed. Results Claudin-7 was down-regulated in UC samples compared to NU samples (P < 0.001). NMI (Ta-T1) tumours had significantly higher claudin-7 expression than MI (≥pT2) tumours (P = 0.012). There was no significant difference between patients with G1-2 tumours and those with G3 tumours (P = 0.19). There was no significant difference between patients with recurrent NMI UC and those with no recurrence (P = 0.61). IHC showed a lower expression of claudin-7 in the UC samples than NU samples, and in MI UC than in NMI UC. Conclusions These results indicate that a reduced expression of claudin-7 correlates with the invasiveness and progression of UC of the urinary bladder. Further studies are needed to validate claudin-7 as a marker for UC. © 2013 Production and hosting by Elsevier B.V. on behalf of Arab Association of Urology.
Authors & Co-Authors
Gadelmoula, Mohamed
Egypt, Asyut
Assiut University
Fukumori, Tomoharu
Japan, Tokushima
Graduate School of Medical Sciences
Nakatsuji, Hiroyoshi
Japan, Tokushima
Graduate School of Medical Sciences
Elgammal, Mohammed Abd Alla
Egypt, Asyut
Assiut University
Toida, Kazunori
Japan, Kurashiki
Kawasaki Medical School
Kanayama, Hiroomi
Japan, Tokushima
Graduate School of Medical Sciences
Statistics
Citations: 6
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.aju.2013.04.006
ISSN:
2090598X
e-ISSN:
20905998
Research Areas
Cancer
Genetics And Genomics