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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Diversity of genetic lineages among CTX-M-15 and CTX-M-14 producing Escherichia coli strains in a Tunisian hospital
Current Microbiology, Volume 62, No. 6, Year 2011
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Description
Fourteen broad-spectrum-cephalosporin-resistant Escherichia coli isolates were recovered between June and December 2007 in a Tunisian hospital. Genes encoding extended-spectrum-beta-lactamases (ESBL) and other resistance genes were characterized by PCR and sequencing. The following ESBL genes were identified: bla CTX-M-15 (12 isolates), bla CTX-M-14a (one isolate), and bla CTX-M-14b (one isolate). The bla OXA-1 gene was detected in 13 bla CTX-M-producing strains and a bla TEM-1 gene in 6 of them. The ISEcp1 sequence was found upstream of bla CTX-M genes in 8 of 14 strains, and orf477 or IS903 downstream of this gene in 13 strains. Nine of the strains carried class 1 integrons and five different gene cassette arrangements were detected, dfrA17-aadA5 being the most common. One of the strains (bla CTX-M-14a-positive) harbored three class 1 integrons, and one of them was non-previously described containing as gene cassettes new variants of aac(6′)-Ib and cmlA1 genes and it was linked to the bla CTX-M-14a gene flanked by a truncated ISEcp1 sequence (included in GenBank with accession number JF701188). CTX-M-15-producing strains were ascribed to phylogroup B2 (six isolates) and D (six isolates). Multilocus-sequence-typing revealed ten different sequence-types (STs) among ESBL-positive E. coli strains with prevalence of ST405 (four strains of phylogroup D) and ST131 types (two strains of phylogroup B2 and serogroup O25b). A high clonal diversity was also observed among studied strains by pulsed-field-gel-electrophoresis (11 unrelated profiles). CTX-M-15 is an emergent mechanism of resistance in the studied hospital and the world-disseminated 0:25b-ST131-B2 and ST405-D clones have been identified among CTX-M-15-producing isolates. © 2011 Springer Science+Business Media, LLC.
Authors & Co-Authors
Slama, Karim Ben
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Ben Sallem, Rym
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Jouini, Ahlem
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Rachid, Salwa
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Moussa, Laurence
Tunisia, Tunis
Laboratoire de Microbiologie
Sáenz, Yolanda
Spain, Logrono
Centro de Investigación Biomédica de la Rioja Cibir
Estepa-Pérez, Vanesa
Spain, Logrono
Universidad de la Rioja
Somalo, Sergio
Spain, Logrono
Universidad de la Rioja
Boudabous, Abdellatif
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Torres, Carmen
Spain, Logrono
Centro de Investigación Biomédica de la Rioja Cibir
Spain, Logrono
Universidad de la Rioja
Statistics
Citations: 52
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1007/s00284-011-9930-4
ISSN:
03438651
e-ISSN:
14320991
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study