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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Limited variation of the 5′cis-control region of the transmission blocking vaccine candidate Pfs25 amid great genetic diversity of Plasmodium falciparum in Cameroon
African Journal of Biotechnology, Volume 7, No. 5, Year 2008
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Description
Genetic recombination during sexual reproduction within Plasmodium sp. contributes to parasite diversity and altered gene expression of certain surface markers. The pfs25 gene involved in the upset of gametocytogenesis is a candidate antigen in transmission blocking vaccine. This study investigated the polymorphism of Pfs25 within its 5′cis-control region in field isolates from different ecotypes in Cameroon. Symptomatic patients and asymptomatic healthy school children with a positive smear and from different ecozones were included. Parasite DNA was extracted and polymorphisms within pfs25, cg2-ω, msp-1, msp-2 and glurp genes were investigated by PCR-RFLP and DNA sequencing. Putative control elements of the 5′cis control regions of Pfs25 were identified by PCGENE software and enzymes were selected whose sequences produced or abolished restriction sites by mutations. Malaria infection was mainly caused by Plasmodium falciparum with sporadic occurrence of Plasmodium malariae and Plasmodium ovale. Analysis of the Pfs25 5′ cis-control region identified only one polymorphism (0.002%) that abolished an RsaI restriction site as part of the sequence TTTCTGTAC, located 40 bp downstream of the promoter and found at - 478 bp of the ATG. Analysis of the 5′ cis-control sequence of Pfs25 revealed minimal variation of the promoter region amid great zonal differences in parasite population. Altitudinal differences in parasite populations were not easily discernable. © 2008 Academic Journals.
Authors & Co-Authors
Mbacham, Wilfred Fon
Cameroon, Yaounde
Université de Yaoundé I
Mimche, Patrice Nsangou
Cameroon, Yaounde
Université de Yaoundé I
Netongo, Palmer Masumbe
Cameroon, Yaounde
Université de Yaoundé I
Evehe, Marie Solange Bebandue
Cameroon, Yaounde
Université de Yaoundé I
Nji, Akindeh Mbuh
Cameroon, Yaounde
Université de Yaoundé I
Amunom, Immaculate
Cameroon, Yaounde
Université de Yaoundé I
Daily, Johanna Patricia
United States, Boston
Harvard T.h. Chan School of Public Health
Makoge, Valérie
Cameroon, Yaounde
Université de Yaoundé I
Laserson, Kayla F.
United States, Boston
Harvard T.h. Chan School of Public Health
Yong, Songmbe Michael
Cameroon, Buea
University of Buea
Lomah, Nicoline
Cameroon, Buea
University of Buea
Enyong, Peter Ayuk I.
Cameroon, Douala
Ministry of Scientific Research
Titanji, Vincent Pryde Kehdinga
Cameroon, Buea
University of Buea
Wirth, Dyann F.
United States, Boston
Harvard T.h. Chan School of Public Health
Statistics
Citations: 14
Authors: 14
Affiliations: 4
Identifiers
e-ISSN:
16845315
Research Areas
Genetics And Genomics
Infectious Diseases
Maternal And Child Health
Sexual And Reproductive Health
Study Design
Cross Sectional Study
Study Locations
Cameroon