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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Phytophthora infestans has a plethora of phospholipase D enzymes including a subclass that has extracellular activity
PLoS ONE, Volume 6, No. 3, Article e17767, Year 2011
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Description
In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol. © 2011 Meijer et al.
Authors & Co-Authors
Meijer, Harold J.G.
Unknown Affiliation
Hassen, Hussen Harrun
Unknown Affiliation
Govers, Francine
Unknown Affiliation
Statistics
Citations: 23
Authors: 3
Affiliations: 4
Identifiers
Doi:
10.1371/journal.pone.0017767
e-ISSN:
19326203