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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Monoclonal antibodies against Enterocytozoon bieneusi of human origin
Clinical and Diagnostic Laboratory Immunology, Volume 12, No. 9, Year 2005
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Description
Enterocytozoon bieneusi is clinically the most significant among the microsporidia infecting humans, causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. The lack of immune reagents is largely due to the absence of methods for laboratory propagation of E. bieneusi. We recently described a procedure for the concentration and purification of spores from diarrheic stool of infected humans. Purified spores were used to immunize mice for production and screening of monoclonal antibodies (MAbs) against E. bieneusi. The eight immunoglobulin M MAbs generated and fully characterized did not cross-react with other human microsporidia or with other microorganisms normally present in stool. One of the MAbs, 2G4, reacted with E. bieneusi spores in stools from monkeys and humans, without background fluorescence, which makes it an ideal diagnostic reagent. It also recognizes intracellular stages of the parasite and will be suitable for determining tissue distribution of E. bieneusi in infected hosts. At least two immunodominant antigens of E. bieneusi of 33,000 and 35,000 Da exist, which were recognized by rabbit and mouse antisera. The availability of MAbs against E. bieneusi will simplify considerably the diagnosis of this infection in humans and will provide tools for epidemiologic investigations regarding the true prevalence of the infection in various human and mammalian populations and the environmental sources of infection. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Sheoran, Abhineet S.
United States, Grafton
Cummings School of Veterinary Medicine
Feng, Xiaochuan
United States, Grafton
Cummings School of Veterinary Medicine
Singh, Inderpal
United States, Grafton
Cummings School of Veterinary Medicine
Chapman-Bonofiglio, Susan
United States, Grafton
Cummings School of Veterinary Medicine
Bakeera-Kitaka, Sabrina
Uganda, Kampala
School of Medicine, Makerere University College of Health Sciences
Hanawalt, Joel
United States, Grafton
Cummings School of Veterinary Medicine
Nunnari, John
United States, Grafton
Cummings School of Veterinary Medicine
Mansfield, Keith G.
United States, Southborough
New England Regional Primate Research Center
Tumwine, James Kashugyera
Uganda, Kampala
School of Medicine, Makerere University College of Health Sciences
Tzipori, Saul R.
United States, Grafton
Cummings School of Veterinary Medicine
Statistics
Citations: 22
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1128/CDLI.12.9.1109-1113.2005
ISSN:
1071412X
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study