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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Increased melanizing activity in Anopheles gambiae does not affect development of Plasmodium falciparum
Proceedings of the National Academy of Sciences of the United States of America, Volume 103, No. 45, Year 2006
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Description
Serpins are central to the modulation of various innate immune responses in insects and are suspected to influence the outcome of malaria parasite infection in mosquito vectors. Three Anopheles gambiae serpins (SRPN1, -2, and -3) were tested for their ability to inhibit the prophenoloxidase cascade, a key regulatory process in the melanization response. Recombinant SRPN1 and -2 can bind and inhibit a heterologous phenoloxidase-activating protease and inhibit phenoloxidase activation in vitro. Using a reverse genetics approach, we studied the effect of SRPN2 on melanization in An. gambiae adult females in vivo. Depletion of SRPN2 from the mosquito hemolymph increases melanin deposition on foreign surfaces such as negatively charged Sephadex beads. As reported, the knockdown of SRPN2 adversely affects the ability of the rodent malaria parasite Plasmodium berghei to invade the midgut epithelium and develop into oocysts. Importantly, we tested whether the absence of SRPN2 from the hemolymph influences Plasmodium falciparum development. RNAi silencing of SRPN2 in an An. gambiae strain originally established from local populations in Yaounde, Cameroon, did not influence the development of autochthonous field isolates of P. falciparum. This study suggests immune evasion strategies of the human malaria parasite and emphasizes the need to study mosquito innate immune responses toward the pathogens they transmit in natural vector-parasite combinations. © 2006 by The National Academy of Sciences of the USA.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC1636544/bin/pnas_0608033103_index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC1636544/bin/pnas_0608033103_1.pdf
Authors & Co-Authors
Michel, Kristin
United Kingdom, London
Imperial College London
Morlais, Isabelle
France, Marseille
Ird Institut de Recherche Pour le Developpement
Lambrechts, Louis
France, Paris
Sorbonne Université
Cohuet, Anna
France, Montpellier
Ird Centre de Montpellier
Awono-Ambéné, Parfait Herman
France, Marseille
Ird Institut de Recherche Pour le Developpement
Simard, Frédéric R.
France, Marseille
Ird Institut de Recherche Pour le Developpement
Fontenille, Didier
France, Montpellier
Ird Centre de Montpellier
Kafatos, Fotis C.
United Kingdom, London
Imperial College London
Statistics
Citations: 88
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1073/pnas.0608033103
ISSN:
00278424
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Cameroon
Participants Gender
Female