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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Studies on tracheorelaxant and anti-inflammatory activities of rhizomes of Polygonatum verticillatum
BMC Complementary and Alternative Medicine, Volume 13, Article 197, Year 2013
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Description
Background: The present study describes the tracheorelaxant and anti-inflammatory effects of Polygonatum verticillatum which may support its medicinal use in hyperactive airway complaints and inflammatory disorders.Methods: The tracheorelaxant activity of crude extract of the rhizomes of P. verticillatum (PR) was assessed in isolated guinea-pig tracheal tissues immersed in tissue organ bath filled with Tyrode's solution and a continuous supply of carbogen gas (95% O2 and 5% CO2). The contractile and relaxant responses of the tissue were measured using isometric transducers coupled with Power-Lab data acquisition system. The anti-inflammatory effect was evaluated in carrageenan-induced rat paw edema model, while the lipoxygenase inhibitory activity was performed in the in-vitro assay. Various chromatographic and spectroscopic techniques were used for the isolation and characterization of pure molecules.Results: In isolated guinea-pig tracheal preparations, PR caused complete inhibition of the high K+ (80 mM) and carbachol-induced contractions however, it was more potent against K+ than CCh, similar to verapamil. Pretreatment of the tissue with PR, displaced the Ca2+ concentration-response curves to the right, similar to that induced by verapamil, indicating the presence of Ca2+ channel blocking like activity. When tested on carrageenan-induced rat paw edema, PR demonstrated a marked reduction in edema with 65.22% protection at 200 mg/kg, similar to aspirin. In the in-vitro assay, PR showed lipoxygenase inhibitory activity (IC50: 102 ± 0.19 μg/mL), similar to baicalein. Bioactivity-guided fractionation led to the isolation of 2-hydroxybenzoic acid and β-sitosterol.Conclusions: These results indicate that the plant possesses tracheorelaxant, mediated possibly through a Ca2+ channel blockade mechanism, and anti-inflammatory activities, which may explain the medicinal use of this plant in airway disorders and inflammation. © 2013 Khan et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Khan, Haroon Ur Rashid
Pakistan, Peshawar
University of Peshawar
Pakistan, Karachi
The Aga Khan University Medical College
Pakistan, Peshawar
Gandhara University
Saeed, Muhammad Muzammal
Pakistan, Peshawar
University of Peshawar
Mehmood, Malik Hassan
Pakistan, Karachi
The Aga Khan University Medical College
Rehman, Najeeb Ur
Pakistan, Karachi
The Aga Khan University Medical College
Pakistan, Dera Ismail Khan
Gomal University
Muhammad, Naveed
Pakistan, Peshawar
University of Peshawar
Pakistan, Mansehra
Hazara University Pakistan
Haq, Ikram Ul
Pakistan, Islamabad
National Institute of Health Pakistan
Ashraf, Nadeem
Canada, London
Robarts Research Institute
El-Tahir, Kamaleldin Eldin H.
Saudi Arabia, Riyadh
College of Pharmacy
Gilani, Anwar ul Hassan
Pakistan, Karachi
The Aga Khan University Medical College
Statistics
Citations: 26
Authors: 9
Affiliations: 8
Identifiers
Doi:
10.1186/1472-6882-13-197
e-ISSN:
14726882
Study Locations
Guinea