Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Screening of ΔF508 mutation and IVS8-poly T polymorphism in CFTR gene in Tunisian infertile men without CBAVD
Andrologia, Volume 44, No. SUPPL.1, Year 2012
Notification
URL copied to clipboard!
Description
It is well established that cystic fibrosis transmembrane conductance regulator gene (CFTR) mutations are involved in congenital bilateral absence of the vas deferens (CBAVD), causing obstructive azoospermia and male infertility. Also, several studies reported a relatively high prevalence of CFTR gene mutations in healthy men presenting reduced sperm quality. In this study, we investigate ΔF508 mutation and IVS8-polyT polymorphism in CFTR gene in Tunisian infertile men without CBAVD. Genetic analyses were performed in 148 infertile patients and 126 fertile individuals. The polymorphic IVS8-polyT tract in CFTR gene was analysed in only 129 infertile patients and 54 individuals of control group. As well, we screened for Y chromosome microdeletions in all infertile patients. No ΔF508 mutation was diagnosed either in infertile patients or in control group. 5T allele of IVS8-polyT tract was found in both infertile men (4.26%) and fertile individuals (8.33%). 5T/5T genotype was observed only in two azoospermic patients without Y microdeletions. The most frequent genotype of IVS8-polyT tract in infertile men and controls was 7T/7T (69.75% and 59.25% respectively). There was no association between IVS8-polyT polymorphism and reduced semen quality. Neither ΔF508 mutation nor 5T allele is involved in pathogenesis of male infertility in Tunisian infertile patients without CBAVD. © 2011 Blackwell Verlag GmbH.
Authors & Co-Authors
Ghorbel, Myriam
Tunisia, Sfax
Faculty of Medicine of Sfax
Baklouti-Gargouri, Siwar
Tunisia, Sfax
Faculty of Medicine of Sfax
Keskes, Rim
Tunisia, Sfax
Faculty of Medicine of Sfax
Sellami-Ben Hamida, A.
Tunisia, Sfax
Faculty of Medicine of Sfax
Feki-Chakroun, Nozha
Tunisia, Sfax
Faculty of Medicine of Sfax
Bahloul, Ali
Tunisia, Sfax
Chu Habib Bourguiba
Fakhfakh, Faiza
Tunisia, Sfax
Faculty of Medicine of Sfax
Ammar-Keskes, Leila
Tunisia, Sfax
Faculty of Medicine of Sfax
Statistics
Citations: 15
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1111/j.1439-0272.2011.01193.x
ISSN:
03034569
e-ISSN:
14390272
Research Areas
Cancer
Genetics And Genomics
Sexual And Reproductive Health
Study Design
Randomised Control Trial
Cross Sectional Study
Participants Gender
Male