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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Application of TaqMan low-density arrays for simultaneous detection of multiple respiratory pathogens
Journal of Clinical Microbiology, Volume 49, No. 6, Year 2011
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Description
The large and growing number of viral and bacterial pathogens responsible for respiratory infections poses a challenge for laboratories seeking to provide rapid and comprehensive pathogen identification. We evaluated a novel application of the TaqMan low-density array (TLDA) cards for real-time PCR detection of 21 respiratory-pathogen targets. The performance of the TLDA was compared to that of individual real-time PCR (IRTP) assays with the same primers and probes using (i) nucleic acids extracted from the 21 pathogen strains and 66 closely related viruses and bacteria and (ii) 292 clinical respiratory specimens. With spiked samples, TLDA cards were about 10-fold less sensitive than IRTP assays. By using 292 clinical specimens to generate 2,238 paired individual assays, the TLDA card exhibited 89% sensitivity (95% confidence interval [CI], 86 to 92%; range per target, 47 to 100%) and 98% specificity (95% CI, 97 to 99%; range per target, 85 to 100%) overall compared to IRTP assays as the gold standard with a threshold cycle (CT) cutoff of 43. The TLDA card approach offers promise for rapid and simultaneous identification of multiple respiratory pathogens for outbreak investigations and disease surveillance. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Conklin, Laura M.
United States, Atlanta
Centers for Disease Control and Prevention
Whitney, Cynthia G.
United States, Atlanta
Centers for Disease Control and Prevention
Anderson, Laurie M.
United States, Atlanta
Centers for Disease Control and Prevention
Schrag, Stephanie J.
United States, Atlanta
Centers for Disease Control and Prevention
Taylor, Thomas H.
United States, Atlanta
Centers for Disease Control and Prevention
Beall, Bernard W.
United States, Atlanta
Centers for Disease Control and Prevention
Breiman, Robert F.
United States, Atlanta
Centers for Disease Control and Prevention
Feikin, Daniel R.
United States, Atlanta
Centers for Disease Control and Prevention
Njenga, Moses Kariuki
United States, Atlanta
Centers for Disease Control and Prevention
Mayer, Leonard W.
United States, Atlanta
Centers for Disease Control and Prevention
Oberste, Mark Steven
United States, Atlanta
Centers for Disease Control and Prevention
Tondella, Maria Lúcia Cecconi
United States, Atlanta
Centers for Disease Control and Prevention
Winchell, Jonas M.
United States, Atlanta
Centers for Disease Control and Prevention
Lindstrom, Stephen E.
United States, Atlanta
Centers for Disease Control and Prevention
Erdman, Dean D.
United States, Atlanta
Centers for Disease Control and Prevention
Fields, Barry S.
United States, Atlanta
Centers for Disease Control and Prevention
Statistics
Citations: 215
Authors: 16
Affiliations: 1
Identifiers
Doi:
10.1128/JCM.02270-10
ISSN:
1098660X