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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
HLA class II induction by interferon-γ in K562 variant cell line: inhibition by serum lipid
Human Immunology, Volume 31, No. 1, Year 1991
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Description
The induction of class II antigen (Ag) by interferon-γ (IFN-γ) in variants of the K562 cell line has been examined in this study. Following incubation of K562A cells with IFN-γ, surface expression of HLA-DR molecules was demonstrated by indirect immunofluorescence, confirmed by immunoblotting, and HLA-DR3 Ag specificity identified. HLA-DP and HLA-DRW52 Ag were co-expressed, but no HLA-DQ expression occurred. A variant of this line, designated K1A, spontaneously developed resistance to IFN-γ induction of class II Ag, but continued to express class I Ag. In a third K562 variant, designated K562B, no class II gene products could be induced. Northern blot analysis indicated that mRNA levels correlated with surface class I and II Ag expression in all of the K562 cell lines. Resista IFN-γ inducible class II Ag expression in K1A cells did not involve changes in IFN-γ receptor affinity or number but was shown to be due to an inhibitory effect of serum lipid. These results indicate that cells derived from a common parental line may differ in susceptibility to a regulatory mechanism affecting IFN-γ inducible class II Ag expression. © 1991.
Authors & Co-Authors
Ress, Stanley R.
South Africa, Cape Town
Faculty of Health Sciences
Rousseau, Jeanne W.
South Africa, Cape Town
Faculty of Health Sciences
Ratanjee, Bharati Dhanluxmi
South Africa, Cape Town
Faculty of Health Sciences
Eidne, Karin Ann
South Africa, Cape Town
Faculty of Health Sciences
Millar, Robert P.
South Africa, Cape Town
Faculty of Health Sciences
Keraan, Mustapha M.
South Africa, Cape Town
Faculty of Health Sciences
Statistics
Citations: 6
Authors: 6
Affiliations: 1
Identifiers
Doi:
10.1016/0198-8859(91)90049-F
ISSN:
01988859
Research Areas
Genetics And Genomics