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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Comparison of PCR-RFLP and genescan-based genotyping for analyzing infection dynamics of Plasmodium falciparum
American Journal of Tropical Medicine and Hygiene, Volume 74, No. 6, Year 2006
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Description
Parameters describing the infection dynamics of Plasmodium falciparum are important determinants of the potential impact of interventions and are potential outcome measurements for malaria intervention trials. Low parasite densities, periodic sequestration of parasites, and the presence of multiple concurrent infections make it essential to use molecular techniques to estimate the force of infection and duration of infections in endemic areas. We now compare two approaches for tracking individual genotypes of the highly polymorphic merozoite surface protein 2: 1) fluorescence-labeled polymerase chain reaction (PCR) and GeneScan-sizing and 2) restriction fragment length polymorphism (RFLP). We analyze samples from a longitudinal field study in Ghana and use statistical approaches that allow for imperfect detectability. The two methods gave broadly similar estimates of parasite dynamics, but GeneScan is more precise and can achieve a higher throughput. The analysis of parasite dynamics indicated an average duration of infection of 210 days by GeneScan versus 152 days by PCR-RFLP in the study population in Kassena-Nankana, Northern Ghana. This reflects the good performance of GeneScan-based genotyping for studies of parasite infection dynamics. Copyright © 2006 by The American Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Falk, Nicole
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Maire, Nicolas
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Sama, Wilson
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Owusu-Agyei, Seth
Ghana, Kintampo
Kintampo Health Research Centre
Smith, Thomas A.
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Beck, Hans Peter
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Felger, Ingrid
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Statistics
Citations: 121
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.4269/ajtmh.2006.74.944
ISSN:
00029637
Research Areas
Health System And Policy
Infectious Diseases
Study Design
Randomised Control Trial
Cross Sectional Study
Cohort Study
Study Locations
Ghana