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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Inhibition of HIV-1 transmission in trans from dendritic cells to CD4
+
T lymphocytes by natural antibodies to the CRD domain of DC-SIGN purified from breast milk and intravenous immunoglobulins
Immunology, Volume 123, No. 4, Year 2008
Notification
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Description
The present study demonstrates that human breast milk and normal human polyclonal immunoglobulins purified from plasma [intravenous immunoglobulins (IVIg)] contain functional natural immunoglobulin A (IgA) and IgG antibodies directed against the carbohydrate recognition domain (CRD) domain of the dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) molecule, which is involved in the binding of human immunodeficiency virus (HIV)-1 to dendritic cells (DCs). Antibodies to DC-SIGN CRD were affinity-purified on a matrix to which a synthetic peptide corresponding to the N-terminal CRD domain (amino-acid 342-amino-acid 371) had been coupled. The affinity-purified antibodies bound to the DC-SIGN peptide and to the native DC-SIGN molecule expressed by HeLa DC-SIGN+ cells and immature monocyte-derived dendritic cells (iMDDCs), in a specific and dose-dependent manner. At an optimal dose of 200 μg/ml, natural antibodies to DC-SIGN CRD peptide purified from breast milk and IVIg stained 25 and 20% of HeLa DC-SIGN+ cells and 32 and 12% of iMDDCs, respectively. Anti-DC-SIGN CRD peptide antibodies inhibited the attachment of virus to HeLa DC-SIGN by up to 78% and the attachment to iMDDCs by only 20%. Both breast milk- and IVIg-derived natural antibodies to the CRD peptide inhibited 60% of the transmission in trans of HIV-1JRCSF, an R5-tropic strain, from iMDDCs to CD4+ T lymphocytes. Taken together, these observations suggest that the attachment of HIV to DCs and transmission in trans to autologous CD4+ T lymphocytes occur through two independent mechanisms. Our data support a role of natural antibodies to DC-SIGN in the modulation of postnatal HIV transmission through breast-feeding and in the natural host defence against HIV-1 in infected individuals. © 2007 Blackwell Publishing Ltd.
Authors & Co-Authors
Requena, Mary
France, Paris
Inserm
Bouhlal, Hicham
France, Paris
Inserm
Nasreddine, Nadine
France, Paris
Inserm
Saïdi, Héla
France, Paris
Inserm
Gody, Jean Chrysostome
Central African Republic
Complexe Pédiatrique
Aubry, Sylvie
France, Paris
Institut de Puériculture et de Périnatalogie
Grésenguet, Gérard
Central African Republic, Bangui
Université de Bangui Faculte Des Sciences de la Sante
Kazatchkine, Michel Daniel
France, Paris
Inserm
Seḱaly, Rafick Pierre
France, Paris
Inserm
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Bélec, Laurent
France, Paris
Inserm
France, Paris
Université Paris Cité
Hocini, Hakim
France, Paris
Inserm
Statistics
Citations: 35
Authors: 11
Affiliations: 6
Identifiers
Doi:
10.1111/j.1365-2567.2007.02717.x
ISSN:
00192805
e-ISSN:
13652567
Research Areas
Infectious Diseases
Maternal And Child Health