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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Quality control program within a clinical trial consortium for PCR protocols to detect plasmodium species
Journal of Clinical Microbiology, Volume 52, No. 6, Year 2014
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Description
Malaria parasite infections that are only detectable by molecular methods are highly prevalent and represent a potential transmission reservoir. The methods used to detect these infections are not standardized, and their operating characteristics are often unknown.We designed a proficiency panel of Plasmodium spp. in order to compare the accuracy of parasite detection of molecular protocols used by labs in a clinical trial consortium. Ten dried blood spots (DBSs) were assembled that contained P. falciparum, P. vivax, P. malariae, and P. ovale; DBSs contained either a single species or a species mixed with P. falciparum. DBS panels were tested in 9 participating laboratories in a masked fashion. Of 90 tests, 68 (75.6%) were correct; there were 20 false-negative results and 2 false positives. The detection rate was 77.8% (49/63) for P. falciparum, 91.7% (11/12) for P. vivax, 83.3% (10/12) for P. malariae, and 70% (7/10) for P. ovale. Most false-negative P. falciparum results were from samples with an estimd <5 parasites perl of blood. Between labs, accuracy ranged from 100% to 50%. In one lab, the inability to detect species in mixed-species infections prompted a redesign and improvement of the assay. Most PCR-based protocols were able to detect P. falciparum and P. vivax at higher densities, but these assays may not reliably detect parasites in samples with low P. falciparum densities. Accordingly, formal quality assurance for PCR should be employed whenever this method is used for diagnosis or surveillance. Such efforts will be important if PCR is to be widely employed to assist malaria elimination efforts. © 2014 American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Taylor, Steve M.
United States, Durham
Duke University Medical Center
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Mayor, A. G.
Spain, Barcelona
Instituto de Salud Global de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Mombo-Ngoma, Ghyslain
Gabon, Lambarene
Centre de Recherches Médicales de Lambaréné
Gabon, Libreville
Universite Des Sciences de la Sante Liberville
Gabon, Fougamou
Ngounie Medical Research Centre
Germany, Tubingen
Eberhard Karls Universität Tübingen
Kenguele Moundounga, Hilaire
Gabon, Lambarene
Centre de Recherches Médicales de Lambaréné
Gabon, Franceville
Universite Des Sciences et Techniques de Masuku
Ouedraogo, Smaïla M.
Benin, Cotonou
University of Abomey-calavi
Burkina Faso, Ouagadougou
Centre Hospitalier Universitaire Yalgado Ouedraogo
Burkina Faso, Ouagadougou
University of Ouagadougou
Tuikue-Ndam, Nicaise
France, Marseille
Ird Institut de Recherche Pour le Developpement
Benin, Cotonou
University of Abomey-calavi
Mkali, Happy
Tanzania, Ifakara
Ifakara Health Institute
Mwangoka, Grace W.
Tanzania, Ifakara
Ifakara Health Institute
Valecha, Neena
India, New Delhi
National Institute of Malaria Research India
Singh, Jai Prakash Narayan
India, New Delhi
National Institute of Malaria Research India
Clark, Martha A.
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Verweij, Jaco J.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Netherlands, Tilburg
St. Elisabeth Ziekenhuis
Adégnika, Ayôla Akim
Gabon, Lambarene
Centre de Recherches Médicales de Lambaréné
Germany, Tubingen
Eberhard Karls Universität Tübingen
Severini, Carlo
Italy, Rome
Istituto Superiore Di Sanita
Menegon, Michela
Italy, Rome
Istituto Superiore Di Sanita
MacEte, Eusébio V.
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Menéndez, Clara
Spain, Barcelona
Instituto de Salud Global de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Cisteró, Pau
Spain, Barcelona
Instituto de Salud Global de Barcelona
Njie, Fanta
Gambia, Banjul
Medical Research Council Laboratories Gambia
Affara, Muna
Gambia, Banjul
Medical Research Council Laboratories Gambia
Otieno, Kephas O.
United States, Atlanta
Centers for Disease Control and Prevention
Kariuki, Simon K.
United States, Atlanta
Centers for Disease Control and Prevention
ter Kuile, Feiko O.
United Kingdom, Liverpool
Liverpool School of Tropical Medicine
Meshnick, Steven Richard
United States, Chapel Hill
The University of North Carolina at Chapel Hill
Statistics
Citations: 35
Authors: 24
Affiliations: 21
Identifiers
Doi:
10.1128/JCM.00565-14
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Infectious Diseases