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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Comparison of three serological tests in the diagnosis of Brucella infection in unvaccinated cattle in Eritrea
Preventive Veterinary Medicine, Volume 48, No. 3, Year 2001
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Description
Three serological methods, the Rose-Bengal test (RBT), the complement-fixation test (CFT) and an indirect enzyme-linked immunosorbent assay (I-ELISA) were compared for the detection of Brucella-infected animals in unvaccinated cattle herds in Eritrea. In this study, 71 herds first were classified as positive or negative for Brucella infection on the basis of at least one animal being seropositive by RBT and CFT. All the 159 RBT-positive samples from the 26 seropositive herds and 214 RBT-negative samples randomly selected from the seropositive herds and from the 45 negative herds were tested further by CFT and I-ELISA. Using the ELISA titer as main predictor, and incorporating the RBT results, a logistic model was built to predict the CFT-negative or -positive status of individual sera and to estimate sensitivity and specificity. Whilst the ELISA titers (≤20) accurately predicted all the negative sera in herds that were also negative by the CFT, the number of seropositive animals was higher by ELISA in herds that had positive animals. Serum samples which give higher degrees of agglutination with the RBT need not be re-tested with CFT; consideration of the seropositive status of a herd should be taken into consideration on defining the cut-off optical density readings for ELISA. © 2001 Elsevier Science B.V.
Authors & Co-Authors
Omer, Mohamed K.
Eritrea, Asmara
University of Asmara
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Skjerve, Eystein
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
MacMillan, A. P.
United Kingdom, Addlestone
Veterinary Laboratory Agency
Woldehiwet, Zerai
United Kingdom, Liverpool
University of Liverpool
Statistics
Citations: 28
Authors: 4
Affiliations: 4
Identifiers
Doi:
10.1016/S0167-5877(00)00185-9
ISSN:
01675877
Study Locations
Eritrea