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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer)
Veterinary Immunology and Immunopathology, Volume 160, No. 1-2, Year 2014
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Description
We assessed the use of Mycobacterium bovis-specific peptides for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer) by evaluating the agreement between the single intradermal comparative tuberculin test (SICTT), the Bovigam® EC (BEC) assay, the Bovigam® HP (BHP) assay and two assays utilizing the QuantiFERON® TB-Gold (in tube) system employing 20h (mQFT20 assay) and 30h (mQFT30 assay) whole blood incubation periods. Of 84 buffaloes, 45% were SICTT-positive, 48% were BEC-positive, 50% were BHP-positive, 37% were mQFT20-positive and 43% were mQFT30-positive. Agreement between the BEC and BHP Bovigam® assays was high (κ=0.86, 95% CI 0.75-0.97) and these detected the most test-positive animals suggesting that they were the most sensitive assays. Interferon-gamma release was significantly greater in buffaloes that were test-positive for all tests than in animals with discordant but positive Bovigam® results. Agreement between the mQFT assays was equally high (κ=0.88, 95% CI 0.77-0.98); however, all buffaloes with discordant mQFT results (n=6) were mQFT30-positive/mQFT20-negative, including three confirmed M. bovis-infected animals, suggesting that the mQFT30 assay is the more sensitive of the two. Agreements between the two Bovigam® and two mQFT assays were moderate, suggesting that in its current format the mQFT assay is less sensitive than either the BEC or the BHP assays. © 2014 Elsevier B.V.
Authors & Co-Authors
Goosen, Wynand J.
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Miller, Michele Ann
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Chegou, Novel N.
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Cooper, David V.
South Africa, Cascade
Ezemvelo Kzn Wildlife
Warren, Robin Mark
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Van Helden, Paul D.
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Parsons, Sven D.C.
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Statistics
Citations: 48
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.vetimm.2014.03.015
ISSN:
01652427
e-ISSN:
18732534
Research Areas
Infectious Diseases