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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
environmental science
Mutagenic and genotoxic assessment of atrazine-based herbicide to freshwater fish Channa punctatus (Bloch) using micronucleus test and single cell gel electrophoresis
Environmental Toxicology and Pharmacology, Volume 31, No. 2, Year 2011
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Description
The mutagenic and genotoxic effect of 'Rasayanzine', an atrazine herbicide, was carried out in fish Channa punctatus using micronucleus and single cell gel electrophoresis (SCGE) assays. Three sub-lethal concentrations of the test material viz., SL-I (1/5th LC 50=∼8.48mgL -1), SL-II (1/8th LC 50=∼5.30mgL -1) and SL-III (1/10th LC 50=∼4.24mgL -1) were calculated using LC 50 value and the fish specimens were exposed to these concentrations. Erythrocyte and gill cells were sampled on day 0, 1, 3, 5, 7, 14, 21, 28 and 35 of exposure for assessment of micronuclei induction in blood erythrocytes and DNA damage using SCGE assay in both blood erythrocytes and gill cells. Significant effects (p<0.01) for both concentration and time of exposure were observed in treated fish. Micronuclei induction in erythrocytes was highest (8.4mgL -1) on day 7 of exposure. The highest level of DNA damage in the SCGE was observed in both tissues on day 5 at all concentrations followed by gradual non-linear decline. This study further confirmed that the micronucleus and SCGE assays are useful in determining potential genotoxicity of water pollutants and might be appropriate as part of monitoring program. © 2011 Elsevier B.V.
Authors & Co-Authors
Nwani, Christopher Didigwu
Nigeria, Abakaliki
Ebonyi State University
Nagpure, Naresh Sahebrao
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Kumar, Ravindra
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Kushwaha, Basdeo
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Kumar, Pavan
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Lakra, Wazir Singh
India, Lucknow
Icar - National Bureau of Fish Genetic Resources, Lucknow
Statistics
Citations: 103
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.etap.2010.12.001
ISSN:
13826689
Research Areas
Environmental
Genetics And Genomics