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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Analysis of the inter- and extracellular formation of platinum nanoparticles by Fusarium oxysporum f. sp. lycopersici using response surface methodology
Nanotechnology, Volume 17, No. 14, Article 021, Year 2006
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Description
Fusarium oxysporum fungal strain was screened and found to be successful for the inter- and extracellular production of platinum nanoparticles. Nanoparticle formation was visually observed, over time, by the colour of the extracellular solution and/or the fungal biomass turning from yellow to dark brown, and their concentration was determined from the amount of residual hexachloroplatinic acid measured from a standard curve at 456 nm. The extracellular nanoparticles were characterized by transmission electron microscopy. Nanoparticles of varying size (10-100 nm) and shape (hexagons, pentagons, circles, squares, rectangles) were produced at both extracellular and intercellular levels by the Fusarium oxysporum. The particles precipitate out of solution and bioaccumulate by nucleation either intercellularly, on the cell wall/membrane, or extracellularly in the surrounding medium. The importance of pH, temperature and hexachloroplatinic acid (H2PtCl6) concentration in nanoparticle formation was examined through the use of a statistical response surface methodology. Only the extracellular production of nanoparticles proved to be statistically significant, with a concentration yield of 4.85 mg l-1 estimated by a first-order regression model. From a second-order polynomial regression, the predicted yield of nanoparticles increased to 5.66 mg l-1 and, after a backward step, regression gave a final model with a yield of 6.59 mg l-1. © 2006 IOP Publishing Ltd.
Authors & Co-Authors
Grewar, T. L.
South Africa, Grahamstown
Rhodes University
Gericke, Mariekie
South Africa, Randburg
Mintek
Whiteley, Chris George
South Africa, Grahamstown
Rhodes University
Statistics
Citations: 239
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1088/0957-4484/17/14/021
ISSN:
09574484
e-ISSN:
13616528