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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Impaired SARS-CoV-2 specific T-cell response in patients with severe COVID-19
Frontiers in Immunology, Volume 14, Article 1046639, Year 2023
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Description
Cellular immune responses are of pivotal importance to understand SARS-CoV-2 pathogenicity. Using an enzyme-linked immunosorbent spot (ELISpot) interferon-γ release assay with wild-type spike, membrane and nucleocapsid peptide pools, we longitudinally characterized functional SARS-CoV-2 specific T-cell responses in a cohort of patients with mild, moderate and severe COVID-19. All patients were included before emergence of the Omicron (B.1.1.529) variant. Our most important finding was an impaired development of early IFN-γ-secreting virus-specific T-cells in severe patients compared to patients with moderate disease, indicating that absence of virus-specific cellular responses in the acute phase may act as a prognostic factor for severe disease. Remarkably, in addition to reactivity against the spike protein, a substantial proportion of the SARS-CoV-2 specific T-cell response was directed against the conserved membrane protein. This may be relevant for diagnostics and vaccine design, especially considering new variants with heavily mutated spike proteins. Our data further strengthen the hypothesis that dysregulated adaptive immunity plays a central role in COVID-19 immunopathogenesis. Copyright © 2023 Rümke, Smit, Bossink, Limonard, Muilwijk, Haas, Reusken, van der Wal, Thio, van Os, Gremmels, Beekman, Nijhuis, Wensing, Heron and Thijsen.
Authors & Co-Authors
Bossink, Ailko W.J.
Netherlands, Utrecht
Diakonessenhuis Utrecht
Haas, Lenneke E.M.
Netherlands, Utrecht
Diakonessenhuis Utrecht
Reusken, Chantal B.E.M.
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Nijhuis, Monique M.
Netherlands, Utrecht
University Medical Center Utrecht
Wensing, Annemarie Marie J.
Netherlands, Utrecht
University Medical Center Utrecht
Statistics
Citations: 1
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.3389/fimmu.2023.1046639
ISSN:
16643224
Research Areas
Covid
Study Design
Cohort Study