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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Acetylcholinesterase biosensor based on a gold nanoparticle-polypyrrole-reduced graphene oxide nanocomposite modified electrode for the amperometric detection of organophosphorus pesticides
Analyst, Volume 139, No. 12, Year 2014
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Description
A nanohybrid of gold nanoparticles, polypyrrole, and reduced graphene oxide sheets (named as Au-PPy-rGO) was achieved by electrochemical deposition of reduced graphene oxide with pyrrole and the introduction of gold nanoparticles. Acetylcholinesterase (AChE) was further encapsulated in a silica matrix and immobilized on the Au-PPy-rGO nanocomposite by co-deposition with (NH4)2SiF6. The presence of PPy helped to avoid the aggregation of rGO caused by van der Waals interactions between individual sheets and significantly increased the surface area of the modified electrode. The obtained Au-PPy-rGO nanocomposite not only showed excellent conductivity but also exhibited a high electrocatalytic activity and specific affinity for thiocholine, the hydrolysis product of the enzyme, and thus an improved detection sensitivity. Since AChE molecules were protected by the circumambient silica matrix, which provided a biocompatible environment and facilitated mass transport, the fabricated AChE biosensor displayed high stability and excellent activity together with a fast response to organophosphorus pesticides. Under optimum conditions, the biosensor led to the rapid and sensitive detection of paraoxon-ethyl from 1.0 nM to 5 μM with a detection limit of 0.5 nM. © 2014 the Partner Organisations.
Authors & Co-Authors
Yang, Yuqi
China, Wuhan
Central China Normal University
Asiri, Abdullah M.
Saudi Arabia, Jeddah
King Abdulaziz University
Du, Dan
China, Wuhan
Central China Normal University
United States, Pullman
Washington State University Pullman
Lin, Yuehe
United States, Pullman
Washington State University Pullman
Statistics
Citations: 153
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1039/c4an00068d
ISSN:
00032654
e-ISSN:
13645528