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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Effects of maneb on testosterone release in male rats
Drug and Chemical Toxicology, Volume 34, No. 2, Year 2011
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Description
Maneb (Manganese ethylene-bis-dithiocarbamate) is a widely used fungicide in agriculture. In order to investigate its effect on male reproductive function, rats were intraperitonealy injected with maneb (1 and 4 mg/kg) for 9 or 18 days. After 6 and 14 days of treatment, the animals received human chorionic gonadotropin (hCG) via a jugular catheter and blood samples were collected at several intervals subsequent to the challenge. They were thereafter decapitated after 9 or 18 days, and organs (i.e., liver, seminal vesicles, and kidneys) were weighed. Leydig cells prepared from rats after 18 days of treatment were incubated with or without different stimulators or precursors [hCG, A23187, 25-OH-cholesterol (25-OH-C), or androstenedione] for 1 hour, and the media were analyzed for testosterone or pregnenolone. Liver glutathione and thiobarbituric acid reactive substances (TBARS) as well as serum alanine aminotransferase (ALT) activity were also measured. Further, Leydig cells and testicular interstitial cells (TICs) prepared from normal rats were incubated with maneb (3-100 μM) for 1 or 2 hours, and testosterone release was assessed. The results showed that administration of maneb (4 mg/kg) for 9 and 18 days did not alter liver function, but resulted in a decrease of basal level of plasma testosterone (P < 0.01). In addition, basal testosterone and pregnenolone release by Leydig cells prepared from maneb 18-day treated animals were significantly reduced (P < 0.05). However, acute in vitro exposure of TIC or Leydig cells to maneb did not alter their testosterone release. These results suggested that maneb alters testosterone production, at least in part, through inhibition of CYP11A1 activitiy. © 2011 Informa Healthcare USA, Inc.
Authors & Co-Authors
TsaguéManfo, Faustin P.
Cameroon, Yaounde
Université de Yaoundé I
France, Villeurbanne
Université Claude Bernard Lyon 1
Chao, Wen Fang
Taiwan, Taipei
Taipei City Hospital Taiwan
Moundipa, Paul Fewou
Cameroon, Yaounde
Université de Yaoundé I
Pugeat, Michel
France, Villeurbanne
Université Claude Bernard Lyon 1
Wang, Paulus S.
Taiwan, Taipei
National Yang-ming University Taiwan
Statistics
Citations: 21
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.3109/01480545.2010.482589
ISSN:
01480545
e-ISSN:
15256014
Research Areas
Health System And Policy
Sexual And Reproductive Health
Participants Gender
Male