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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Regionally-specified second trimester fetal neural stem cells reveals differential neurogenic programming
PLoS ONE, Volume 9, No. 9, Article e105985, Year 2014
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Description
Neural stem/progenitor cells (NSC) have the potential for treatment of a wide range of neurological diseases such as Parkinson Disease and multiple sclerosis. Currently, NSC have been isolated only from hippocampus and subventricular zone (SVZ) of the adult brain. It is not known whether NSC can be found in all parts of the developing mid-trimester central nervous system (CNS) when the brain undergoes massive transformation and growth. Multipotent NSC from the mid-trimester cerebra, thalamus, SVZ, hippocampus, thalamus, cerebellum, brain stem and spinal cord can be derived and propagated as clonal neurospheres with increasing frequencies with increasing gestations. These NSC can undergo multi-lineage differentiation both in vitro and in vivo, and engraft in a developmental murine model. Regionally-derived NSC are phenotypically distinct, with hippocampal NSC having a significantly higher neurogenic potential (53.6%) over other sources (range of 0%-27.5%, p,0.004). Whole genome expression analysis showed differential gene expression between these regionally-derived NSC, which involved the Notch, epidermal growth factor as well as interleukin pathways. We have shown the presence of phenotypically- distinct regionally-derived NSC from the mid-trimester CNS, which may reflect the ontological differences occurring within the CNS. Aside from informing on the role of such cells during fetal growth, they may be useful for different cellular therapy applications. © 2014 Fan et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4152177/bin/pone.0105985.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC4152177/bin/pone.0105985.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC4152177/bin/pone.0105985.s003.docx
Authors & Co-Authors
Fan, Yiping
Singapore, Singapore City
Kk Women's and Children's Hospital
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Marcy, Guillaume
Singapore, Singapore City
Duke-nus Medical School
Lee, Eddy S.M.
United States, Stanford
Richard M. Lucas Center for Imaging
Rozen, Steven George
Singapore, Singapore City
Duke-nus Medical School
Mattar, Citra Nz
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Waddington, Simon N.
United Kingdom, London
University College London
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
Goh, Eyleen L.K.
Singapore, Singapore City
Duke-nus Medical School
Choolani, Mahesh A.
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Chan, Jerry Kok Yen
Singapore, Singapore City
Kk Women's and Children's Hospital
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Singapore, Singapore City
Duke-nus Medical School
Statistics
Citations: 11
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0105985
e-ISSN:
19326203
Research Areas
Cancer
Genetics And Genomics