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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Characterization of Ni-resistant bacteria in the rhizosphere of the hyperaccumulator Alyssum murale by 16S rRNA gene sequence analysis
World Journal of Microbiology and Biotechnology, Volume 26, No. 1, Year 2010
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Description
The diversity of 184 isolates from rhizosphere and bulk soil samples taken from the Ni hyperaccumulator Alyssum murale, grown in a Ni-rich serpentine soil, was determined by 16S rRNA gene analysis. Restriction digestion of the 16S rRNA gene was used to identify 44 groups. Representatives of each of these groups were placed within the phyla Proteobacteria, Firmicutes and Actinobacteria by 16S rRNA gene sequence analysis. By combining the 16S rRNA gene restriction data with the gene sequence analysis it was concluded that 44.6% (82/184) of the isolates were placed within the phylum Proteobacteria, among these 35.9% (66/184) were placed within the class α-Proteobacteria, and 20.7% (38/184) had 16S rRNA gene sequences indicative of bacteria within genera that form symbioses with legumes (rhizobia). Of the remaining isolates, 44.6% (82/184) and 5.4% (10/184) were placed within the phyla Actinobacteria and Firmicutes, respectively. No placement was obtained for a small number (10/184) of the isolates. Bacteria of the phyla Proteobacteria and Actinobacteria were the most numerous within the rhizosphere of A. murale and represented 32.1% (59/184) and 42.9% (79/184) of all isolates, respectively. The approach of using 16S rRNA gene sequence analysis in this study has enabled a comprehensive characterization of bacteria that predominate in the rhizosphere of A. murale growing in Ni-contaminated soil. © Springer Science+Business Media B.V. 2009.
Authors & Co-Authors
Abou-Shanab, Reda A.I.
Egypt, New Borg el Arab
Genetic Engineering and Biotechnology Research Institute
van Berkum, Peter B.
United States, Washington, D.c.
Usda Agricultural Research Service
Angle, Jay Scott
United States, Athens
College of Agricultural and Environmental Sciences Athens Campus
Delorme, T. A.
United States, Kent
Kent State University
Chaney, R. L.
United States, Washington, D.c.
Usda Agricultural Research Service
Ghozlan, Hanan A.
Egypt, Alexandria
Faculty of Science
Ghanem, K. M.
Egypt, Alexandria
Faculty of Science
Moawad, Hassan
Egypt, Giza
National Research Centre
Statistics
Citations: 24
Authors: 8
Affiliations: 6
Identifiers
Doi:
10.1007/s11274-009-0148-6
ISSN:
09593993
Research Areas
Genetics And Genomics