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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Factors affecting the stability of detergent-solubilized cholinephosphotransferase and ethanolaminephosphotransferase
Molecular Membrane Biology, Volume 10, No. 1, Year 1993
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Description
Cholinephosphotransferase (CPT) and ethanolaminephosphotransferase (EPT) are the enzymes catalyzing the last step of the de novo pathway for phosphatidylcholine and phosphatidylethanolamine synthesis, respectively. A major limitation for the complete characterization of the reactions catalyzed by the two enzymes derives from their poor stability in detergent-containing buffers. CPT is heavily inactivated, when native membranes are solubilized using a series of detergents, whereas EPT activity is better preserved during solubilization. An investigation of the factors which could play a role in preserving both enzymes from inactivation was carried out. The dramatic loss of enzymatic activities occurring upon dilution of solubilized membranes with detergent-containing buffers can be reduced by supplementing the dilution medium with phospholipids. The addition of Mn2+ ions to the dispersion buffer increases the stability of both enzymes. The procedure previously described for solubilizing EPT from rat brain microsomes has been modified on the basis of this evidence. Microsomes were solubilized in buffered detergent solutions containing Mn2+ ions and both CPT and EPT were partially purified in their active form by onion-exchange chromatography. © 1993 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Authors & Co-Authors
Mancini, Alessandra
Italy, Perugia
Università Degli Studi Di Perugia, Facoltà Di Medicina e Chirurgia
Roberti, Rita
Italy, Perugia
Università Degli Studi Di Perugia, Facoltà Di Medicina e Chirurgia
Binaglia, Luciano
Italy, Perugia
Università Degli Studi Di Perugia, Facoltà Di Medicina e Chirurgia
Missiri, Amr El
Egypt, Mansoura
Faculty of Science
Freysz, Louis
France, Strasbourg
Centre de Neurochimie
Statistics
Citations: 5
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.3109/09687689309150251
ISSN:
09687688