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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Evaluation of the Roche Cobas Taqman and Abbott realtime extraction-quantification systems for HIV-1 subtypes
Journal of Acquired Immune Deficiency Syndromes, Volume 44, No. 5, Year 2007
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Description
OBJECTIVES: We conducted a comparison of the Abbott Molecular RealTime (Rungis, France) and Roche Diagnostics Cobas Taqman (Meylan, France) automated nucleic acid extraction and real-time polymerase chain reaction (PCR) amplification systems for their capacity to quantify HIV RNA of various subtypes. The systems were tested on culture supernatants belonging to HIV-1 group M (n = 29), HIV-1 group O (n = 8), and HIV-2 (n = 7). We also tested 88 plasma samples from patients infected with HIV-1 group M (B-D [n = 7], A-CRF01 [n = 16], CRF02 [n = 49], and other strains [n = 16]). RESULTS: The Abbott RealTime system quantified all 29 HIV-1 group M supernatants. One of these samples was not detected by the Roche Cobas TaqMan system. The Abbott RealTime system quantified 7 HIV-1 group O strains. Neither technique cross-reacted with HIV-2. The 79% intraclass correlation coefficient for the 88 plasma samples was barely acceptable, but 4 plasma samples were underestimated by more than 1 log by the Roche Cobas TaqMan system. Similar values were obtained for subtype B and D strains with the tests, indicating that the primers and probes are suitable for these strains. In contrast, the large differences observed with other subtypes, particularly CRF02, show the importance of primer and probe selection. CONCLUSION: The limitation of real-time PCR to span the entire diversity of HIV must be taken into account during treatment monitoring, resistance studies, and clinical trials. © 2007 Lippincott Williams & Wilkins, Inc.
Authors & Co-Authors
Gueudin, Marie
France, Caen
Gram 2.0 - Groupe de Recherche Sur L'adaptation Microbienne
France, Rouen
Hopital Charles Nicolle
Plantier, Jean Christophe
France, Caen
Gram 2.0 - Groupe de Recherche Sur L'adaptation Microbienne
Lémée, Véronique
France, Caen
Gram 2.0 - Groupe de Recherche Sur L'adaptation Microbienne
Schmitt, Marie Paule
France, Strasbourg
Les Hôpitaux Universitaires de Strasbourg
Chartier, Loïc
France, Paris
Institut Pasteur, Paris
Bourlet, Thomas
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
Damond, Florence
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Vray, Muriel M.
France, Paris
Institut Pasteur, Paris
Simon, Franćois
France, Caen
Gram 2.0 - Groupe de Recherche Sur L'adaptation Microbienne
France, Strasbourg
Les Hôpitaux Universitaires de Strasbourg
France, Paris
Institut Pasteur, Paris
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
France, Rennes
Hôpital Pontchaillou
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
France, Paris
Hôpital Saint-louis
Statistics
Citations: 137
Authors: 9
Affiliations: 8
Identifiers
Doi:
10.1097/QAI.0b013e31803260df
ISSN:
15254135
Research Areas
Infectious Diseases