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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The variable N-terminal region of DDX5 contains structural elements and auto-inhibits its interaction with NS5B of hepatitis C virus
Biochemical Journal, Volume 446, No. 1, Year 2012
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Description
RNA helicases of the DEAD (Asp-Glu-Ala-Asp)-box family of proteins are involved in many aspects of RNA metabolism from transcription to RNA decay, but most of them have also been shown to be multifunctional. The DEAD-box helicase DDX5 of host cells has been shown to interact with the RNA-dependent RNA polymerase (NS5B) of HCV (hepatitis C virus). In the present study, we report the presence of two independent NS5B-binding sites in DDX5, one located at the N-terminus and another at the C-terminus. The N-terminal fragment of DDX5, which consists of the first 305 amino acids and shall be referred as DDX5-N, was expressed and crystallized. The crystal structure shows that domain 1 (residues 79-303) of DDX5 contains the typical features found in the structures of other DEAD-box helicases. DDX5-N also contains the highly variable NTR (N-terminal region) of unknown function and the crystal structure reveals structural elements in part of the NTR, namely residues 52-78. This region forms an extensive loop and an α-helix. From co-immunoprecipitation experiments, the NTR of DDX5-N was observed to auto-inhibit its interaction with NS5B. Interestingly, the α-helix in NTR is essential for this auto-inhibition and seems to mediate the interaction between the highly flexible 1-51 residues in NTR and the NS5B-binding site in DDX5-N. Furthermore, NMR investigations reveal that there is a direct interaction between DDX5 and NS5B in vitro. © The Authors Journal compilation © 2012 Biochemical Society.
Authors & Co-Authors
Dutta, Sujit
Singapore, Singapore City
A-star, Institute of Molecular and Cell Biology
Singapore, Singapore City
Ngee Ann Polytechnic
Gupta, Garvita
Singapore, Singapore City
National University of Singapore
Choi, Yook Wah
Singapore, Singapore City
A-star, Institute of Molecular and Cell Biology
South Africa, Bellville
University of the Western Cape
Kotaka, Masayo
Hong Kong
The University of Hong Kong Li ka Shing Faculty of Medicine
Fielding, Burtram Clinton
South Africa, Bellville
University of the Western Cape
Song, Jianxing
Singapore, Singapore City
National University of Singapore
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Tan, Yee Joo
Singapore, Singapore City
A-star, Institute of Molecular and Cell Biology
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Statistics
Citations: 12
Authors: 7
Affiliations: 6
Identifiers
Doi:
10.1042/BJ20120001
ISSN:
02646021
e-ISSN:
14708728
Research Areas
Infectious Diseases