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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Monitoring and source tracking of tetracycline resistance genes in lagoons and groundwater adjacent to swine production facilities over a 3-year period
Applied and Environmental Microbiology, Volume 73, No. 15, Year 2007
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Description
To monitor the dissemination of resistance genes into the environment, we determined the occurrence of tetracycline resistance (Tcr) genes in groundwater underlying two swine confinement operations. Monitoring well networks (16 wells at site A and 6 wells at site C) were established around the lagoons at each facility. Groundwater (n = 124) and lagoon (n = 12) samples were collected from the two sites at six sampling times from 2000 through 2003. Total DNA was extracted, and PCR was used to detect seven Tcr genes [tet(M), tet(O), tet(Q), tet(W), tet(C), tet(H), and tet(Z)]. The concentration of Tcr genes was quantified by real-time quantitative PCR. To confirm the Tcr gene source in groundwater, comparative analysis of tet(W) gene sequences was performed on groundwater and lagoon samples. All seven Tcr genes were continually detected in groundwater during the 3-year monitoring period at both sites. At site A, elevated detection frequency and concentration of Tcr genes were observed in the wells located down-gradient of the lagoon. Comparative analysis of tet(W) sequences revealed that the impacted groundwater contained gene sequences almost identical (99.8% identity) to those in the lagoon, but these genes were not found in background libraries. Novel sequence clusters and unique indigenous resistance gene pools were also found in the groundwater. Thus, antibiotic resistance genes in groundwater are affected by swine manure, but they are also part of the indigenous gene pool. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Koike, Satoshi
United States, Urbana
University of Illinois Urbana-champaign
Japan, Sapporo
Hokkaido University
Yannarell, Anthony C.
United States, Urbana
University of Illinois Urbana-champaign
Mackie, Roderick Ian
United States, Urbana
University of Illinois Urbana-champaign
Statistics
Citations: 292
Authors: 3
Affiliations: 5
Identifiers
Doi:
10.1128/AEM.00665-07
ISSN:
00992240
Research Areas
Genetics And Genomics
Health System And Policy
Study Approach
Quantitative