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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Contribution to in vitro screening of Egyptian plants for schistosomicidal activity
Pharmaceutical Biology, Volume 50, No. 6, Year 2012
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Description
Context: This study is a continuation of our previous work in which a bioassay screening of 346 methanol extracts from 281 Egyptian plant species was carried out for in vitro schistosomicidal activity. Objective: Another 309 methanol extracts from 278 plant species were subjected to the bioassay screening using the same technique on viable Schistosoma mansoni Sambon (Schistosomatidae) mature worms in specialized culture medium (Roswell Park Memorial Institute medium 1640) in a trial to discover a source for a schistosomiasis drug from Egyptian flora. Material and methods: The methanol plant extracts were tested in vitro against viable S. mansoni mature worms in culture medium. Viability of worms was examined after exposure to 100 μg/ml of the extract in the medium for 24h. Negative (dimethyl sulfoxide) and positive (praziquantel) controls were simultaneously used. Extracts showing schistosomicidal activity were further subjected to determination of their (Lethal concentration) LC50 and LC90 values. Results: Confirmed in vitro antischistosomal activity was found in 42 extracts. Of these, 14 plant species possessed considerably high antischistosomal activity (LC 50 ≤ 15 μg/ml), viz. Callistemon viminalis (Soland. Ex Gaertn) Cheel, C. rigidus R.Br., C. speciosus (Sims.) DC, C. citrinus Stapf, Eucalyptus citriodora Hook, E. rostrata Dehnh., Eugenia edulis Vell, E. javanica Lam syn. Syzygium samarangense (Blume) Merril, Melaleuca leucadendron (L.) L., M. stypheloides Sm. (all belong to Myrtaceae), Cryptostegia grandiflora R.Br. (Asclepiadaceae), Zilla spinosa (L.) Prantl (Cruciferae), Ficus trijuja L. (Moraceae) and Fagonia mollis Delile (Zygophylacae). Discussion and conclusion: These species may represent additional natural sources of bioactive material that deserve further investigation for drug discovery against schistosomiasis. © 2012 Informa Healthcare USA, Inc.
Authors & Co-Authors
Yousif, Fouad
Egypt, Giza
Theodor Bilharz Research Institute
Wassel, Gamila M.
Egypt, Giza
National Research Centre
Boulos, Loutfy
Egypt, Alexandria
Faculty of Science
Labib, Therese
Egypt, Giza
Orman Botanical Garden
Mahmoud, Khaled H.
Egypt, Giza
National Research Centre
El-Hallouty, Salwa M.
Egypt, Giza
National Research Centre
El-Bardicy, Samia N.
Egypt, Giza
Theodor Bilharz Research Institute
Mahmoud, Soheir Sayed M.
Egypt, Giza
Theodor Bilharz Research Institute
Ramzy, F.
Egypt, Giza
Theodor Bilharz Research Institute
Gohar, Lamiaa
Egypt, Giza
National Research Centre
El-Manawaty, M. A.
Egypt, Giza
National Research Centre
El Gendy, Mohamed A.M.
Egypt, Giza
National Research Centre
Fayad, Walid
Egypt, Giza
National Research Centre
El-Menshawi, Bassem S.
Egypt, Giza
National Research Centre
Statistics
Citations: 28
Authors: 14
Affiliations: 4
Identifiers
Doi:
10.3109/13880209.2011.625952
ISSN:
13880209
e-ISSN:
17445116
Research Areas
Health System And Policy
Infectious Diseases