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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Replication modes of Maize streak virus mutants lacking RepA or the RepA-pRBR interaction motif
Virology, Volume 442, No. 2, Year 2013
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Description
The plant-infecting mastreviruses (family Geminiviridae) express two distinct replication-initiator proteins, Rep and RepA. Although RepA is essential for systemic infectivity, little is known about its precise function. We therefore investigated its role in replication using 2D-gel electrophoresis to discriminate the replicative forms of Maize streak virus (MSV) mutants that either fail to express RepA (RepA-), or express RepA that is unable to bind the plant retinoblastoma related protein, pRBR. Whereas amounts of viral DNA were reduced in two pRBR-binding deficient RepA mutants, their repertoires of replicative forms changed only slightly. While a complete lack of RepA expression was also associated with reduced viral DNA titres, the only traces of replicative intermediates of RepA- viruses were those indicative of recombination-dependent replication. We conclude that in MSV, RepA, but not RepA-pRBR binding, is necessary for single-stranded DNA production and efficient rolling circle replication. © 2013 Elsevier Inc.
Authors & Co-Authors
Ruschhaupt, Moritz
Germany, Stuttgart
Universität Stuttgart
Martin, Darren Patrick
South Africa, Cape Town
University of Cape Town
Lakay, Francisco M.
South Africa, Cape Town
University of Cape Town
Bezuidenhout, Marion E.
South Africa, Cape Town
University of Cape Town
Rybicki, Edward P.
South Africa, Cape Town
University of Cape Town
Jeske, Holger
Germany, Stuttgart
Universität Stuttgart
Shepherd, Dionne Natalie
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 22
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.virol.2013.04.012
ISSN:
00426822
e-ISSN:
10960341
Research Areas
Genetics And Genomics