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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
In vitro evaluation of endothelial cell loss using the neusidl corneal inserter
Cornea, Volume 32, No. 4, Year 2013
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Description
PURPOSE:: To determine the immediate endothelial cell loss (ECL) resulting from insertion of a precut donor button using the Neusidl Corneal Inserter (NCI) and compare it with the previously published ECL resulting from insertion of a folded donor button using non-coapting forceps. METHODS:: Ten corneas were precut for Descemet stripping automated endothelial keratoplasty and trephinated to a diameter of 8.0 mm (n = 5) or 8.5 mm (n = 5). Each tissue was placed onto the platform of a new NCI spatula and inserted into a cadaveric whole globe through a 5.2 mm incision. The tissue was carefully removed and stained with trypan blue and alizarin red to detect damaged endothelium. ECL was estimated using Adobe Photoshop planimetry. Mean ECL was compared with previously reported studies of forceps insertion with a one-sample t test, using SPSS v. 19. Geographic patterns of ECL were also documented. RESULTS:: Mean ECL was 15.6% (95% confidence interval, 13.8-17.4). We were unable to detect a difference in ECL compared with previous insertion methods studied (P < 0.001). The pattern of damage from the NCI was different than that previously seen with forceps insertion. CONCLUSION:: Immediate endothelial damage resulting from use of the NCI for insertion of Descemet stripping automated endothelial keratoplasty tissue is comparable with that seen with a standard forceps technique, but with a different damage pattern. Copyright © 2012 by Lippincott Williams Wilkins.
Authors & Co-Authors
Davis-Boozer, David D.
Portugal
Devers Eye Institute
Terry, Mark A.
Portugal
Devers Eye Institute
Greiner, Mark A.
Portugal
Devers Eye Institute
Holiman, Jeffrey D.
Unknown Affiliation
Saad, Hisham A.
Egypt, Tanta
Faculty of Medicine
Alqudah, Asem A.
Portugal
Devers Eye Institute
Li, Jennifer Y.
United States, Sacramento
Uc Davis Health
Statistics
Citations: 8
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1097/ICO.0b013e318274a72b
e-ISSN:
15364798
Study Approach
Quantitative