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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Advanced yellow fever virus genome detection in point-of-care facilities and reference laboratories
Journal of Clinical Microbiology, Volume 50, No. 12, Year 2012
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Description
Reported methods for the detection of the yellow fever viral genome are beset by limitations in sensitivity, specificity, strain detection spectra, and suitability to laboratories with simple infrastructure in areas of endemicity. We describe the development of two different approaches affording sensitive and specific detection of the yellow fever genome: a real-time reverse transcription-quantitative PCR (RT-qPCR) and an isothermal protocol employing the same primer-probe set but based on helicase-dependent amplification technology (RT-tHDA). Both assays were evaluated using yellow fever cell culture supernatants as well as spiked and clinical samples. We demonstrate reliable detection by both assays of different strains of yellow fever virus with improved sensitivity and specificity. The RT-qPCR assay is a powerful tool for reference or diagnostic laboratories with real-time PCR capability, while the isothermal RT-tHDA assay represents a useful alternative to earlier amplification techniques for the molecular diagnosis of yellow fever by field or point-of-care laboratories. Copyright © 2012 American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3503008/bin/supp_50_12_4054__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3503008/bin/JCM.01799-12_zjm999092142so1.pdf
Authors & Co-Authors
Domingo, C. Tiffany
Germany, Berlin
Robert Koch Institute
Patel, Pranav
Germany, Berlin
Robert Koch Institute
Yillah, Jasmin
Germany, Berlin
Robert Koch Institute
Weidmann, Manfred W.
Germany, Gottingen
Universitätsmedizin Göttingen
Méndez, Jairo A.
Colombia, Bogota
Instituto Nacional de Salud
Nakouné, Emmanuel Rivalyn
Central African Republic, Bangui
Institut Pasteur de Bangui
Niedrig, Matthias
Germany, Berlin
Robert Koch Institute
Statistics
Citations: 149
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1128/JCM.01799-12
ISSN:
00951137
e-ISSN:
1098660X
Research Areas
Infectious Diseases
Study Approach
Quantitative