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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Laccase-catalyzed dimerization of ferulic acid amplifies antioxidant activity
Journal of Molecular Catalysis B: Enzymatic, Volume 74, No. 1-2, Year 2012
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Description
Enzymatic modification can be used to enhance the bioactive properties of phenolic compounds. The present study employed laccase from Trametes pubescens to catalyze the modification of ferulic acid in a monophasic or biphasic system, as a way of enhancing its antioxidant capacity. Two dimeric products (m/z 385.1) were purified and characterized as the β-5 and β-β dimers. In the monophasic system, the β-5 dimer was preferentially formed in dioxane while the β-β dimer formation was enriched in ethanol as co-solvent. In the biphasic system, formation of the dimers increased as the concentration of ethyl acetate was increased from 80% to 95%. The β-5 dimer showed higher antioxidant capacity than the substrate as demonstrated by standard antioxidant assays (DPPH and TEAC). These results demonstrate that alteration of reaction conditions influences the laccase-mediated oxidation of ferulic acid to form dimers with higher antioxidant capacity than the substrate. .© 2011 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Adelakun, Oluyemisi Elizabeth
South Africa, Bellville
Cape Peninsula University of Technology
Kudanga, Tukayi
South Africa, Bellville
Cape Peninsula University of Technology
Parker, Ayesha
South Africa, Bellville
Cape Peninsula University of Technology
Green, Ivan Robert
South Africa, Bellville
University of the Western Cape
Le Roes-Hill, Marilize
South Africa, Bellville
Cape Peninsula University of Technology
Burton, Stephanie G.
South Africa, Bellville
Cape Peninsula University of Technology
Statistics
Citations: 74
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1016/j.molcatb.2011.08.010
ISSN:
13811177
e-ISSN:
18733158