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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Mining the genetic diversity of Ehrlichia ruminantium using map genes family
Veterinary Parasitology, Volume 167, No. 2-4, Year 2010
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Description
Understanding bacterial genetic diversity is crucial to comprehend pathogenesis. Ehrlichia ruminantium (E. ruminantium), a tick-transmitted intracellular bacterial pathogen, causes heartwater disease in ruminants. This model rickettsia, whose genome has been recently sequenced, is restricted to neutrophils and reticulo-endothelial cells of its mammalian host and to the midgut and salivary glands of its vector tick. E. ruminantium harbors a multigene family encoding for 16 outer membrane proteins including MAP1, a major antigenic protein. All the 16 map paralogs are expressed in bovine endothelial cells and some are specifically translated in the tick or in the mammalian host. In this study, we carried out phylogenetic analyses of E. ruminantium using sequences of 6 MAP proteins, MAP1, MAP1-2, MAP1-6, MAP1-5, MAP1+1 and MAP1-14, localized either in the center or at the borders of the map genes cluster. We show that (i) map1 gene is a good tool to characterize the genetic diversity among Africa, Caribbean islands and Madagascar strains including new emerging isolates of E. ruminantium; (ii) the different map paralogs define different genotypes showing divergent evolution; (iii) there is no correlation between all MAP genotypes and the geographic origins of the strains; (iv) The genetic diversity revealed by MAP proteins is conserved whatever is the scale of strains sampling (village, region, continent) and thus was not related to the different timing of strains introduction, i.e. continuous introduction of strains versus punctual introduction (Africa versus Caribbean islands). These results provide therefore a significant advance towards the management of E. ruminantium diversity. The differential evolution of these paralogs suggests specific roles of these proteins in host-vector-pathogen interactions that could be crucial for developing broad-spectrum vaccines. © 2009 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Raliniaina, Modestine
Madagascar, Antananarivo
Fofifa-drzv
France, Petit-bourg
Centre de Recherche Antilles-guyane
Meyer, Damien F.
France, Petit-bourg
Centre de Recherche Antilles-guyane
Pinarello, Valérie
France, Petit-bourg
Centre de Recherche Antilles-guyane
Sheikboudou, Christian
France, Petit-bourg
Centre de Recherche Antilles-guyane
Emboulé, Loic
France, Petit-bourg
Centre de Recherche Antilles-guyane
Kandassamy, Yane
France, Petit-bourg
Centre de Recherche Antilles-guyane
Adakal, Hassane
Burkina Faso, Bobo-dioulasso
Centre International de Recherche-developpement Sur Lelevage en Zone Subhumide, Bobo-dioulasso
Stachurski, Frédéric
France, Montpellier
Inrae's Occitanie-montpellier Centre
Burkina Faso, Bobo-dioulasso
Centre International de Recherche-developpement Sur Lelevage en Zone Subhumide, Bobo-dioulasso
Martinez, Dominique
France, Montpellier
Inrae's Occitanie-montpellier Centre
Lefrancois, T.
France, Petit-bourg
Centre de Recherche Antilles-guyane
Vachiéry, Nathalie
France, Petit-bourg
Centre de Recherche Antilles-guyane
Statistics
Citations: 28
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1016/j.vetpar.2009.09.020
ISSN:
03044017
Research Areas
Environmental
Genetics And Genomics
Study Locations
Madagascar