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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Identification of a fibronectin-binding domain within the Campylobacter jejuni CadF protein
Molecular Microbiology, Volume 57, No. 4, Year 2005
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Description
The binding of Campylobacter jejuni to fibronectin (Fn), a component of the extracellular matrix, is mediated by a 37 KDa outer membrane protein termed CadF for Campylobacter adhesion to Fn. Previous studies have indicated that C. jejuni binds to Fn on the basolateral surface of T84 human colonic cells. To further characterize the interaction of the CadF protein with Fn, enzyme-linked immunosorbent assays were performed to identify the Fn-binding domain (Fn-BD). Using overlapping 30-mer and 16-mer peptides derived from translated cadF nucleotide sequence, maximal Fn-binding activity was localized to four amino acids (AA 134-137) consisting of the residues phenylalanine-arginine-leucine- serine (FRLS). A mouse α-CadF peptide polyclonal antibody (M α-CadF peptide pAb) was generated using FRLS containing peptides and found to react with viable C. jejuni as judged by indirect fluorescent microscopy, suggesting that the FRLS residues are surface-exposed. Binding of CadF to purified Fn and INT 407 human epithelial cells was significantly inhibited with peptides containing the Fn-BD. Moreover, a CadF recombinant variant protein, in which the Phe-Arg-Leu residues (CadF AA 134-136) were altered to Ala-Ala-Gly, exhibited a 91% decrease in Fn-binding activity as compared with the wild-type CadF protein. Collectively, these data indicate that the FRLS residues (CadF AA 134-137) of the C. jejuni CadF protein possess Fn-binding activity. © 2005 Blackwell Publishing Ltd.
Authors & Co-Authors
Konkel, Michael E.
United States, Pullman
Washington State University Pullman
Christensen, Jeffrey E.
United States, Pullman
Washington State University Pullman
Keech, Amy M.
United States, Pullman
Washington State University Pullman
Monteville, Marshall R.
United States, Pullman
Washington State University Pullman
Egypt, Cairo
Us Naval Medical Research Unit
Klena, John David
United States, Pullman
Washington State University Pullman
Egypt, Cairo
Us Naval Medical Research Unit
Garvis, Steve G.
United States, Pullman
Washington State University Pullman
France, Paris
Cnrs Centre National de la Recherche Scientifique
Statistics
Citations: 106
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1111/j.1365-2958.2005.04744.x
ISSN:
0950382X