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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Isolation of Cryptococcus gattii and Cryptococcus neoformans var. grubii from the flowers and bark of Eucalyptus trees in India
Medical Mycology, Volume 43, No. 6, Year 2005
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Description
The association of Cryptococcus gattii with Eucalyptus trees has been well established. Here we report the isolation of both C gattii and Cryptococcus neoformans var. grubii from the flowers and bark of Eucalyptus trees in India. We investigated a total of 233 samples of Eucalyptus trees: 120 flowers, 81 fragments of bark, and 32 leaves. C gattii was isolated from two samples of flowers of Eucalyptus terreticornis. C. neoformans var. grubii was recovered twice from the bark of Eucalyptus camaldulensis, initially from one of three samples, and again 2 months later, from one of four samples collected beneath the canopy of the tree. The primary isolation medium was Nigerseed agar, and brown colonies were presumptively identified as C. gattii or C. neoformans. The species identification was confirmed by morphological and biochemical characteristics. Using the Crypto-Check kit (Iatron, Tokyo, Japan), the first two isolates were identified as serotype B (C. gattii) and the other two were serotype A (C. neoformans var. grubii). PCR analysis of the isolates of C. neoformans var. grubii revealed that they possessed the MATα mating type allele. Molecular typing by amplified fragment length polymorphism markers indicated that both isolates of C. neoformans var. grubii possessed the same genotype. This study demonstrates that C. neoformans var. grubii, as well as C. gattii, may be associated with Eucalyptus trees. © 2005 ISHAM.
Authors & Co-Authors
Gugnani, Harish Chander C.
India, New Delhi
University of Delhi
Mitchell, Thomas G.
United States, Durham
Duke University Medical Center
Litvintseva, Anastasia P.
United States, Durham
Duke University Medical Center
Statistics
Citations: 51
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1080/13693780500160785
ISSN:
14602709
Research Areas
Genetics And Genomics