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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Distribution of DArT, AFLP, and SSR markers in a genetic linkage map of a doubled-haploid hexaploid wheat population
Genome, Volume 49, No. 5, Year 2006
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Description
A genetic linkage mapping study was conducted in 93 doubled-haploid lines derived from a cross between Triticum aestivum L. em. Thell 'Arina' and a Norwegian spring wheat breeding line, NK93604, using diversity arrays technology (DArT), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. The objective of this study was to understand the distribution, redundancy, and segregation distortion of DArT markers in comparison with AFLP and SSR markers. The map contains a total of 624 markers with 189 DArTs, 165 AFLPs and 270 SSRs, and spans 2595.5 cM. All 3 marker types showed significant (p < 0.01) segregation distortion, but it was higher for AFLPs (24.2%) and SSRs (22.6%) than for DArTs (13.8%). The overall segregation distortion was 20.4%. DArTs showed the highest frequency of clustering (27.0%) at < 0.5 cM intervals between consecutive markers, which is 3 and 15 times higher than SSRs (8.9%) and AFLPs (1.8%), respectively. This high proportion of clustering of DArT markers may be indicative of gene-rich regions and (or) the result of inclusion of redundant clones in the genomic representations, which was supported by the presence of very high correlation coefficients (r > 0.98) and multicollinearity among the clustered markers. The present study is the first to compare the utility of DArT with AFLP and SSR markers, and the present map has been successfully used to identify novel QTLs for resistance to Fusarium head blight and powdery mildew and for anther extrusion, leaf segment incubation, and latency. © 2006 NRC.
Authors & Co-Authors
Semagn, Kassa
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Björnstad, Åsmund
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Skinnes, Helge
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Marøy, Anne Guri
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Tarkegne, Yalew
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
William, H. M.
Mexico, Texcoco
Centro Internacional de Mejoramiento de Maiz y Trigo
Statistics
Citations: 140
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1139/G06-002
ISSN:
08312796
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study