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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Genetic diversity assessment of summer squash landraces using molecular markers
Molecular Biology Reports, Volume 40, No. 7, Year 2013
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Description
Plant identification, classification, and genotyping within a germplasm collection are essential elements for establishing a breeding program that enhances the probability of plants with desirable characteristics in the market place. In this study, random amplified polymorphic DNA (RAPD) was used as a molecular tool to assess the diversity and relationship among 20 summer squash (Curcubita pepo L.) landraces traditionally used to treat hypertension and prostate hyperplasia. A total of 10 RAPD primers produced 65 reproducible bands of which 46 (70.77 %) were polymorphic, indicating a large number of genotypes within the summer squash lines. Cluster analysis divided the summer squash germplasm into two groups, one including one landrace and a second containing 19 landraces that could be divided into five sub-groups. Results of this study indicate the potential of RAPD markers for the identification and assessment of genetic variations among squash landraces and provide a number of choices for developing a successful breeding program to improve summer squash. © 2013 Springer Science+Business Media Dordrecht.
Authors & Co-Authors
Mady, Emad
United States, Amherst
University of Massachusetts Amherst
Egypt, Cairo
Faculty of Agriculture
Helaly, Alaa Al Din
Egypt, Cairo
Faculty of Agriculture
Abu El-Hamd, Abdel Naem
Egypt, Cairo
Faculty of Agriculture
Abdou, Arafa
Egypt, Cairo
Faculty of Agriculture
Shanan, Shamel A.
Egypt, Cairo
Faculty of Agriculture
Craker, Lyle Eugene
United States, Amherst
University of Massachusetts Amherst
Statistics
Citations: 14
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1007/s11033-013-2510-x
ISSN:
03014851
e-ISSN:
15734978
Research Areas
Genetics And Genomics
Noncommunicable Diseases