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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A comparison of two DNA probes, one specific for Plasmodium falciparum and one with wider reactivity, in the diagnosis of malaria
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 84, No. 2, Year 1990
Notification
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Description
The sensitivity and specificity of 2 probes for the detection of malarial infection was studied. 399 blood samples from Gambian children were tested in a deoxyribonucleic acid (DNA) hybridization assay, and the results compared with the microscopical findings from thick blood films. 8 additional pure Plasmodium malariae and 14 pure P. vivax samples were also assayed. One probe, containing a 21 base pair tandem repeat and highly specific for P. falciparum, detected this species in all except 2 of 74 samples with a parasitaemia of 250 per or more; the overall sensitivity of the probe was 76%. The other probe, a 6 kilobase pair organelle DNA, is conserved in all Plasmodium species so far tested. Its sensitivity for P. falciparum was lower than the 21 base pair repeat, but it detected P. vivax and P. malariae at low levels of parasitaemia, and thus could be useful in field studies. © 1990 Royal Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Holmberg, Martin
Sweden, Stockholm
Karolinska Institutet
Sweden, Uppsala
Uppsala Universitet
Vaidya, Akhil B.
United States, Philadelphia
Drexel University College of Medicine
Shenton, F. C.
Gambia, Banjul
Medical Research Council Laboratories Gambia
Snow, Robert William
Gambia, Banjul
Medical Research Council Laboratories Gambia
Greenwood, Brian M.
Gambia, Banjul
Medical Research Council Laboratories Gambia
Wigzell, Hans L.
Sweden, Stockholm
Karolinska Institutet
Pettersson, Ulf
Sweden, Uppsala
Uppsala Universitet
Statistics
Citations: 7
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1016/0035-9203(90)90255-D
ISSN:
00359203
e-ISSN:
18783503
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Maternal And Child Health