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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
African origin of GB virus C determined by phylogenetic analysis of a complete genotype 5 genome from South Africa
Journal of General Virology, Volume 86, No. 6, Year 2005
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Description
GB virus C (GBV-C), a positive-strand RNA virus, currently infects approximately one-sixth of the world's population. This virus exists as a collection of genotypes whose global distribution correlates with geographical origin. Genotyping of GBV-C isolates by phylogenetic analysis has relied upon the use of 5′-untranslated region (5′-UTR) sequences, however, complete genome sequences are used to demonstrate definitively their existence and geographical correlation. Initial identification of the fifth genotype from South Africa was based upon phylogenetic analysis of the 5′-UTR. It was sought to confirm this classification by analysis of full-length E2 genes from South African isolates and by analysis of a complete genotype 5 genome. Analysis of full-length E2 genes from 28 GBV-C-infected South African individuals revealed the existence of a unique group of 18 isolates, distinct from the other four genotypes. Bootstrap analysis provided strong support (95%) for this fifth group. The remaining isolates were either genotype 1 (n = 8) or 2 (n = 2). Analysis of human E2 gene sequences, with the E2 gene from the chimpanzee variant GBV-Ctro) included as the outgroup, produced a tree rooted on the genotype 1 branch. The complete genome nucleotide sequence of South African genotype 5 isolate D50 was determined. Phylogenetic analysis of the 5′-UTR and open reading frame produced congruent trees that grouped the sequences into five major genotypes. Inclusion of the corresponding region of the chimpanzee isolate GBV-Ctro in the analysis produced trees rooted on the branch leading to the genotype 5 isolate D50, suggesting an ancient African origin of GBV-C. © 2005 SGM.
Authors & Co-Authors
Muerhoff, Anthony Scott
United States, Santa Clara
Abbott Diagnostics
Leary, Thomas P.
United States, Santa Clara
Abbott Diagnostics
Sathar, Mahomed Aslam
South Africa, Durban
The Nelson R. Mandela Medical School
Dawson, George J.
United States, Santa Clara
Abbott Diagnostics
Desai, Suresh M.
United States, Santa Clara
Abbott Diagnostics
Statistics
Citations: 31
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1099/vir.0.80854-0
ISSN:
00221317
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
South Africa