Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
General contribution of nonspecific interactions to fluorescence intensity
Analytical Chemistry, Volume 78, No. 11, Year 2006
Notification
URL copied to clipboard!
Description
Many chemical compounds, including nonfluorescent ones, induce changes in the fluorescence spectra of certain probes, such as berberine cation and Reichardt's betaine, both in the absence and the presence of solvent, that affect almost exclusively emission intensity. In this work, the application of fluorescence detection by intensity changes (FDIC) to HPLC and TLC chromatographic systems with fluorescence detectors has been studied. FDIC detection is of special interest in detecting nonfluorescent analytes, either in HPLC or in TLC mode. It does not involve covalent interactions, and the dielectric permittivity (ε) of the medium plays an important role. The balance between nonspecific and specific interactions produces either an increase or a decrease in fluorescence intensity. Therefore, the influence of chromatographic conditions and chemical structure of analytes on the sign and magnitude of fluorescence peaks for sample detection in HPLC and TLC systems has been discussed. In general, probe nature and concentration determine response and detection sensitivity for a given sample in TLC and HPLC. As solubility and fluorescence properties in solvents determine the operating conditions for a FDIC probe in HPLC mode, nature and flows of mobile phase and solvent are important for chromatographic response and detection sensitivity. © 2006 American Chemical Society.
Authors & Co-Authors
Gálvez, Eva M.
Spain, Zaragoza
Csic - Instituto de Carboquimica Icb
Matt, Muriel
France, Nancy
Université de Lorraine
Cebolla, Vicente L.
Spain, Zaragoza
Csic - Instituto de Carboquimica Icb
Fernandes, Francisco
France, Nancy
Université de Lorraine
Membrado, Luis
Spain, Zaragoza
Csic - Instituto de Carboquimica Icb
Cossío, Fernando P.
Spain, Leioa
Universidad Del Pais Vasco
Garriga, Rosa
Spain, Zaragoza
Universidad de Zaragoza
Vela, Jesús
Spain, Zaragoza
Universidad de Zaragoza
Guermouche, Moulay Hassane
Algeria, Algiers
Université Des Sciences et de la Technologie Houari Boumediene
Statistics
Citations: 20
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1021/ac058045b
Research Areas
Environmental