Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Primary culture of Mycobacterium ulcerans from human tissue specimens after storage in semisolid transport medium
Journal of Clinical Microbiology, Volume 46, No. 1, Year 2008
Notification
URL copied to clipboard!
Description
Tissue specimens collected from patients with clinically suspected Buruli ulcer treated in two Buruli ulcer treatment centers in Benin between 1998 and 2004 were placed in semisolid transport medium and transported at ambient temperature for microbiological analysis at the Institute of Tropical Medicine in Antwerp, Belgium. The impact of the delay before microbiological analysis on primary culture of Mycobacterium ulcerans was investigated. The length of storage in semisolid transport medium varied from 6 days to 26 weeks. Of the 1,273 tissue fragments positive for M. ulcerans DNA by an IS2404-specific PCR, 576 (45.2%) yielded positive culture results. The sensitivity of direct smear examination was 64.6% (822/1,273 tissue fragments). The median time required to obtain a positive culture result was 11 weeks. Positive cultures were obtained even from samples kept for more than 2 months at ambient temperatures. Moreover, there was no reduction in the viability of M. ulcerans, as detected by culture, when specimens remained in semisolid transport medium for long periods of time (up to 26 weeks). We can conclude that the method with semisolid transport medium is very robust for clinical specimens from patients with Buruli ulcer that, due to circumstances, cannot be analyzed in a timely manner. This transport medium is thus very useful for the confirmation of a diagnosis of Buruli ulcer with specimens collected in the field. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Eddyani, Miriam
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Debacker, Martine
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Martin, Anandi
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Aguiar, Julia
Benin
Centre Sanitaire et Nutritionel Gbemoten
Johnson, Christian Roch
Benin
Programme National de Lutte Contre L'ulcère de Buruli
Uwizeye, Cécile
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Fissette, Kristina
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Portaels, Françoise Ç.Oise
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Statistics
Citations: 52
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1128/JCM.00301-07
ISSN:
00951137
Research Areas
Genetics And Genomics
Health System And Policy
Study Locations
Benin