Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Functional analysis of members of the isoflavone and isoflavanone O-methyltransferase enzyme families from the model legume Medicago truncatula
Plant Molecular Biology, Volume 62, No. 4-5, Year 2006
Notification
URL copied to clipboard!
Description
Previous studies have identified two distinct O-methyltransferases (OMTs) implicated in isoflavonoid biosynthesis in Medicago species, a 7-OMT methylating the A-ring 7-hydroxyl of the isoflavone daidzein and a 4′-OMT methylating the B-ring 4′-hydroxyl of 2,7,4′-trihydroxyisoflavanone. Genes related to these OMTs from the model legume Medicago truncatula cluster as separate branches of the type I plant small molecule OMT family. To better understand the possible functions of these related OMTs in secondary metabolism in M. truncatula, seven of the OMTs were expressed in E. coli, purified, and their in vitro substrate preferences determined. Many of the enzymes display promiscuous activities, and some exhibit dual regio-specificity for the 4′ and 7-hydroxyl moieties of the isoflavonoid nucleus. Protein structure homology modeling was used to help rationalize these catalytic activities. Transcripts encoding the different OMT genes exhibited differential tissue-specific and infection- or elicitor-induced expression, but not always in parallel with changes in expression of confirmed genes of the isoflavonoid pathway. The results are discussed in relation to the potential in vivo functions of these OMTs based on our current understanding of the phytochemistry of M. truncatula, and the difficulties associated with gene annotation in plant secondary metabolism. © 2006 Springer Science+Business Media B.V.
Authors & Co-Authors
Deavours, Bettina E.
United States, Ardmore
Samuel Roberts Noble Foundation
United States, Fort Collins
Colorado State University
Liu, Chang Jun
United States, Chevy Chase
Howard Hughes Medical Institute
United States, Upton
Brookhaven National Laboratory
Naoumkina, Marina A.
United States, Ardmore
Samuel Roberts Noble Foundation
Tang, Yuhong
United States, Ardmore
Samuel Roberts Noble Foundation
Farag, Mohamed Ali
United States, Ardmore
Samuel Roberts Noble Foundation
Egypt, Cairo
Faculty of Pharmacy
Sumner, Lloyd W.
United States, Ardmore
Samuel Roberts Noble Foundation
Noel, Joseph Patrick
United States, Chevy Chase
Howard Hughes Medical Institute
Dixon, Richard Arthur
United States, Ardmore
Samuel Roberts Noble Foundation
Statistics
Citations: 50
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1007/s11103-006-9050-x
ISSN:
01674412
Research Areas
Genetics And Genomics