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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Ultra‐rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase‐polymerase chain reaction
Journal of Medical Virology, Volume 40, No. 2, Year 1993
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Description
A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties of guanidinium thiocyanate in the presence of silica. The silica RT‐PCR can be completed within 5 hours starting from RNA extraction to agarose gel electrophoresis. All of the 63 dengue‐3 culture‐positive sera were RT‐PCR positive (virus titres: <102 to 1110.69). Of 33 culture‐ negative acute sera from serologically confirmed dengue fever patients collected during dengue‐3 epidemic, 4 were RT‐PCR‐positive. RT‐PCR was also positive in 29 of 30 dengue‐1 culture‐ positive sera (virus titres range: <102 to 108.69). Dengue‐1 virus was also detected in fieldcaught Aedes aegypti mosquitoes by silica RT‐PCR. © 1993 Wiley‐Liss, Inc. Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company
Authors & Co-Authors
Chungue, Eliane
French Polynesia, Papeete
Institut Territorial de Recherches Medicales Louis Malarde
Barbazan, Philippe
French Polynesia, Papeete
Institut de Recherche Pour le Developpement Papeete
Chanteau, Suzanne
French Polynesia, Papeete
Institut Territorial de Recherches Medicales Louis Malarde
Statistics
Citations: 62
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1002/jmv.1890400211
ISSN:
01466615
Research Areas
Infectious Diseases