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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Maternal serum protein profile and immune response protein subunits as markers for non-invasive prenatal diagnosis of trisomy 21, 18, and 13
Prenatal Diagnosis, Volume 33, No. 3, Year 2013
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Description
Objectives: To use proteomics to identify and characterize proteins in maternal serum from patients at high-risk for fetal trisomy 21, trisomy 18, and trisomy 13 on the basis of ultrasound and maternal serum triple tests. Methods: We performed a comprehensive proteomic analysis on 23 trisomy cases and 85 normal cases during the early second trimester of pregnancy. Protein profiling along with conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis/Tandem mass spectrometry analysis was carried out to characterize proteins associated with each trisomy condition and later validated using Western blot. Results: Protein profiling approach using surface enhanced laser desorption/ionization time-of-flight mass (SELDI-TOF/MS) spectrometry resulted in the identification of 37 unique hydrophobic proteomic features for three trisomy conditions. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by Matrix Assisted Laser Desorption Ionization - Time of Flight/Time of Flight (MALDI-TOF/TOF) and western blot, glyco proteins such as alpha-1-antitrypsin, apolipoprotein E, apolipoprotein H, and serum carrier protein transthyretin were identified as potential maternal serum markers for fetal trisomy condition. The identified proteins showed differential expression at the subunit level. Conclusions: Maternal serum protein profiling using proteomics may allow non-invasive diagnostic testing for the most common trisomies and may complement ultrasound-based methods to more accurately determine pregnancies with fetal aneuploidies. © 2013 John Wiley & Sons, Ltd.
Authors & Co-Authors
Narasimhan, Kothandaraman
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Saudi Arabia, Jeddah
King Abdulaziz University
Lin, Su Lin
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Tong, Terry
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Baig, Sonia
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Ho, Sherry
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Sukumar, Ponnusamy
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Biswas, Arijit
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Hahn, Sinuhe
Switzerland, Basel
Universitätsspital Basel
Bajic, Vladimir B.
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Choolani, Mahesh A.
Singapore, Singapore City
Nus Yong Loo Lin School of Medicine
Statistics
Citations: 16
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1002/pd.4047
ISSN:
01973851
e-ISSN:
10970223
Research Areas
Maternal And Child Health
Sexual And Reproductive Health