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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Evaluation of viremia frequencies of a novel human pegivirus by using bioinformatic screening and PCR
Emerging Infectious Diseases, Volume 22, No. 4, Year 2016
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Description
Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses. © 2016, Centers for Disease Control and Prevention (CDC). All rights reserved.
Authors & Co-Authors
Bonsall, David G.
United Kingdom, Oxford
University of Oxford
Gregory, William F.
United Kingdom, Edinburgh
The University of Edinburgh
Ip, Camilla L.C.
United Kingdom, Oxford
University of Oxford
Donfield, Sharyne M.
United States, Chapel Hill
Rho, Inc.
Iles, James C.
United Kingdom, Oxford
University of Oxford
Ansari, M. Azim
United Kingdom, Oxford
University of Oxford
Piazza, Paolo A.
United Kingdom, Oxford
University of Oxford
Trebes, Amy
United Kingdom, Oxford
University of Oxford
Brown, Anthony
United Kingdom, Oxford
University of Oxford
Frater, John
United Kingdom, Oxford
University of Oxford
Pybus, Oliver George
United Kingdom, Oxford
University of Oxford
Goulder, Philip Jeremy Renshaw
United Kingdom, Oxford
University of Oxford
Klenerman, Paul
United Kingdom, Oxford
University of Oxford
Bowden, Rory J.
United Kingdom, Oxford
University of Oxford
Gomperts, Edward D.
United States, Los Angeles
Children's Hospital Los Angeles
Barnes, Eleanor J.
United Kingdom, Oxford
University of Oxford
Kapoor, Amit
United States, Columbus
Research Institute at Nationwide Childrens Hospital
Sharp, Colin P.
United Kingdom, Edinburgh
The University of Edinburgh
Simmonds, Peter N.
United Kingdom, Oxford
University of Oxford
Statistics
Citations: 29
Authors: 19
Affiliations: 5
Identifiers
Doi:
10.3201/eid2204.151812
ISSN:
10806040
Research Areas
Infectious Diseases
Study Design
Cohort Study
Case-Control Study