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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Pretreatment of defatted wheat germ proteins (by-products of flour mill industry) using ultrasonic horn and bath reactors: Effect on structure and preparation of ACE-inhibitory peptides
Ultrasonics Sonochemistry, Volume 20, No. 6, Year 2013
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Description
The ultrasonic horn and bath reactors were compared based on production of angiotensin-converting-enzyme (ACE) inhibitory peptides from defatted wheat germ proteins (DWGP). The DWGP was sonicated before hydrolysis by Alcalase. Degree of hydrolysis, ACE-inhibitory activity, surface hydrophobicity, fluorescence intensity, free sulfhydryl (SH), and disulfide bond (SS) were determined. The highest ACE-inhibitory activity of DWGP hydrolysate was obtained at power intensity of 191.1 W/cm2 for 10 min in the ultrasonic horn reactor. The fixed frequency of 33 kHz and the sweep frequency of 40 ± 2 kHz resulted in the maximum ACE-inhibitory activity. The combined irradiation of dual fixed frequency (24/68 kHz) produced significant increase in ACE-inhibitory activity compared with single frequency (33 kHz). The ultrasonic probe resulted in significant higher ACE-inhibitory activity compared with ultrasonic bath operating at single or dual fixed and sweep frequencies. The changes in conformation of the DWGP due to sonication were confirmed by the changes in fluorescence intensity, surface hydrophobicity, SHf and SS contents and they were found in conformity with the ACE-inhibitory activity in case of the ultrasonic horn reactor but not in bath reactor. © 2013 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Zhou, Cunshan
China, Zhenjiang
Jiangsu University
Ma, Haile
China, Zhenjiang
Jiangsu University
Yu, Xiaojie
China, Zhenjiang
Jiangsu University
Liu, Bin
China, Beijing
Chinese Academy of Agricultural Mechanization Sciences
Yagoub, Abu El Gasim Ahmed
China, Zhenjiang
Jiangsu University
Sudan, Zalingei
University of Zalingei
Pan, Zhongli J.
China, Zhenjiang
Jiangsu University
United States, Davis
University of California, Davis
Statistics
Citations: 109
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/j.ultsonch.2013.04.005
ISSN:
13504177
e-ISSN:
18732828