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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Characterization and functional investigation of an Arabidopsis cDNA encoding a homologue to the d-PGMase superfamily
Journal of Experimental Botany, Volume 56, No. 414, Year 2005
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Description
An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase super-family: cofactor-dependent phosphoglycerate mutases (d-PGM-ases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Preliminary phylogenetic studies indicated that At-74 cDNA and its close homologue in Arabidopsis, At-74H, belong, however, to an equally distinct group. At-74 was ubiquitously expressed in vegetative organs and induced by glucose. The At-74 cDNA was overexpressed in A. thaliana to investigate its function, but this overexpression did not result in a clear phenotype. Enzymatic assays performed on At-74-overproducing transgenic plants or E. coli cells showed no increase in either the activities of cofactor-dependent and -independent phosphoglycerate mutases (i-PGMases) and F2,6Pase or that of acid phosphatases. The possible role of At-74 in plant metabolism was further investigated by carbon partitioning experiments with [U-14C] glucose and measurements of soluble sugars in both young leaves and roots. Two overexpressing At-74 lines showed a clear increase in glucose uptake. This paper introduces the At-74 homologue of the d-PGMase superfamily members and supports a possible role of At-74 in carbohydrate metabolism. © The Author [2005]. Published by Oxford University Press. All rights reserved.
Authors & Co-Authors
Bourgis, Fabienne
Belgium, Brussels
Université Libre de Bruxelles
Belgium, Ghent
Universiteit Gent
France, Montpellier
Ird Centre de Montpellier
Botha, F. C.
South Africa, Stellenbosch
Stellenbosch University
South Africa, Mount Edgecombe
South African Sugarcane Research Institute
Mani, Srikrishnan
Belgium, Ghent
Universiteit Gent
Belgium, Ghent
Innogenetics
Hiten, Fletcher N.
South Africa, Stellenbosch
Stellenbosch University
Rigden, Daniel John
Brazil, Brasilia
Embrapa Recursos Geneticos e Biotecnologia
Verbruggen, Nathalie
Belgium, Brussels
Université Libre de Bruxelles
Belgium, Ghent
Universiteit Gent
Statistics
Citations: 12
Authors: 6
Affiliations: 7
Identifiers
Doi:
10.1093/jxb/eri105
ISSN:
00220957
Research Areas
Environmental
Genetics And Genomics